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Title |
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Comparative characterization of commercially important xylanase enzymes
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Authors |
Neelima Arora, Amit Kumar Banerjee, Srilaxmi Mutyala, U. S. N Murty*
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Affiliation |
Bioinformatics Group, Biology Division, Indian Institute of Chemical Technology, Hyderabad-500007, A.P., India
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Article Type |
Hypothesis
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Date
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Received April 30, 2009; accepted June 07, 2009; published August 05, 2009
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Abstract
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Xylanase is an industrially important enzyme having wide range of applications especially in paper industry. It is crucial to gain an understanding about the structure and functional aspects of various xylanases produced from diverse sources. In this study, a bioinformatics and molecular modeling approach was adopted to explore properties and structure of Xylanases. Physico-chemical properties were predicted and prediction of motifs, disulfide bridges and secondary structure was performed for functional characterization. Apart from these analyses, three dimensional structures were constructed and stereo-chemical quality was evaluated by different structure validation tools. Comparative catalytic site analysis and assessment was performed to extract information about the important residues. Asn72 was found to be the common residue in the active sites of the proteins P35809 and Q12603.
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Keywords |
Xylanase, Bioinformatics, sequence analysis, structure prediction; function
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Citation |
Arora et al., Bioinformation 3(10): 446-453 (2009)
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Edited by |
P. Kangueane
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ISSN |
0973-2063
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Publisher |
Biomedical Informatics | |
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License
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This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.
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