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Title
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A molecular model for diacylglycerol acyltransferase from Mortierella ramanniana var. angulispora
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Authors
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Sanjay Mishra1,*, Surya Prakash Dwivedi1, Neeraja Dwivedi1, Ajay Kumar1, Anil Rawat2 and Yasushi Kamisaka3
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Affiliation |
1Department of Biotechnology, College of Engineering and Technology, IFTM Campus, Lodhipur-Rajput, Delhi Road, Moradabad 244 001, U.P., India; 2Bioinformatics Laboratory, Biobranz Institute, Lucknow 226 001, U.P., India; 3Lipid Engineering Research Group Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki 305-8566 Japan
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Article Type
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Hypothesis | |
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Date
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received December 10, 2008; revised February 19 2009; accepted April 14, 2009; published June 28, 2009 | |
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Abstract |
Acyl Co-A diacylglycerol acyltransferase (DGAT, EC 2.3.120) is recognized as a key player of cellular diacylglycerol metabolism. It catalyzes the terminal, yet the committed step in triacylglycerol synthesis using diacylglycerol and fatty acyl CoA as substrates. The protein sequence of diacylglycerol acyltransferse (DGAT) Type 2B in Moretierella ramanniana var. angulispora (Protein_ID = AAK84180.1) was retrieved from GenBank. However, a structure is not yet available for this sequence. The 3D structure of DGAT Type 2B was modeled using a template structure (PDB ID: 1K30) obtained from Protein databank (PDB) identified by searching with position specific iterative BLAST (PSI-BLAST). The template (PDB ID: 1K30) describes the structure of DGAT from Cucurbita moschata. Modeling was performed using Modeller 9v2 and protein model is hence generated. The DGAT type 2B protein model was subsequently docked with six inhibitors (sphingosine; trifluoroperazine; phosphatidic acid; lysophospatidylserine; KCl; 1, 2-diolein) using AutoDock (a molecular docking program). The binding of inhibitors to the protein model of DGAT type 2B is discussed.
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Keywords |
AutoDock; DGAT; diacylglycerol (DG); Mortierella ramanniana; Modeller; PDB; phosphatidic acid (PA); PSIBLAST;RMSD; triacylglycerol (TG) | |
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Citation
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Mishra et al, Bioinformation 3(9): 394-398 (2009) | |
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Edited by |
P. Kangueane | |
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ISSN |
0973-2063
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Publisher |
Biomedical Informatics | |
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License
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This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.
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