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Title
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Identification of Comamonas species using 16S rRNA gene sequence
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Authors
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Vimlesh Yadav1, Satya Prakash1, Shipra Srivastava1, Praveen Chandra Verma2, 3, Vijayta Gupta3, Vaishali Basu3 and Anil Kumar Rawat1,*
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Affiliation
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1Biotechnology and Bioinformatics Division, BIOBRAINZ, 566/29 J, Jai Prakash Nagar, Alambagh, Lucknow 226005, U.P., India; 2Department of Biotechnology, Madhav Institute of Technology & Science, Gwaliar (MP), 3Present address: National Botanical Research Institute, Rana Pratap Marg, Lucknow, U.P. India
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Phone |
91- 0522 4042284
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Article Type
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Hypothesis
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Date
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received March 05, 2009; accepted May 16, 2009; published June 13, 2009
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Abstract
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A bacterial strain Bz02 was isolated from a water sample collected from river Gomti at the Indian city of Lucknow. We characterized the strain using 16S rRNA sequence. Phylogenetic analysis showed that the strain formed a monophyletic clade with members of the genus Comamonas. The closest phylogenetic relative was Comamonas testosteroni with 95% 16S rRNA gene sequence similarity. It is proposed that the identified strain Bz02 be assigned as the type strain of a species of the genus Comamonas (Comamonas sp Bz02) based on 16S rRNA gene sequence search in Ribosomal Database Project, small subunit rRNA and large subunit rRNA databases together with the phylogenetic tree analysis. The sequence is deposted in GenBank with the accession number FJ211417.
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Keywords |
16S ribosomal RNA gene; Comamonas; PCR; Phylogenetic analysis; DNA isolation; DNA Sequencing
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Citation |
Yadav et al, Bioinformation 3(9): 381-383 (2009)
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Edited by |
P. Kangueane
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ISSN |
0973-2063
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Publisher |
Biomedical Informatics
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License
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This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License. |