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Title

 

 

 

 

Computational and molecular characterization of multiple isoforms of lfe-2 gene in nematode C. elegans

 

Authors

Luv Kashyap1, Mohammad Tabish1*, Subramaniam Ganesh2, and Deepti Dubey2

Affiliation

1Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh, India; 2Department of Biological Sciences and Bioengineering, Indian Institute of Technology, Kanpur, India

 

Email

tabish.biochem@gmail.com; * Corresponding author

 

Phone

+91-571-270-0741

 

Article Type

Hypothesis

 

Date

received May 01, 2007; accepted May 02, 2007; published online May 30, 2007
 

Abstract

C. elegans C46H11.4 gene encodes a Let-23 fertility effector/regulator protein of the EGF-receptor class of the tyrosine kinase family. Alternative splicing is a major mechanism of generating protein diversity in higher eukaryotes. C. elegans genome sequencing consortium has reported three alternatively spliced transcripts of C46H11.4 gene which encodes for three hypothetical proteins namely, C46H11.4a, C46H11.4b and C46H11.4c. Using a combination of various bioinformatics tools like gene/exon finding programmes, blast searches, alignment tools etc followed by experimental validation, we report the presence of three more alternatively spliced transcripts which encode for novel hypothetical proteins C46H11.4d, C46H11.4e and C46H11.4f. These isoforms arise as a result of alternative splicing in the pre-mRNA encoded by gene C46H11.4. These novel un-reported spliced variants not only point towards the extent of alternative splicing in C. elegans genes but also hint towards the complex nature of alternative splicing.

 

Keywords

 

lfe-2; Let-23 fertility effector/regulator protein; exon prediction; alternative splicing; C. elegans

 

Citation

Kashyap et al., Bioinformation 2(1): 17-21 (2007)

 

Edited by

P. Kangueane

 

ISSN

0973-2063

 

Publisher

Biomedical Informatics

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.