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Title

Anti-quorum sensing activity of Boerhavia diffusa against Pseudomonas aeruginosa PAO1

 

Authors

V Shravani1, 2,*, AS Smiline Girija2*, Himabindu3, Madhan Krishnan4 & Shyamaladevi Babu4

 

Affiliation

1Department of Microbiology, School of Allied Health Sciences, Mallareddy University, Hyderabad, Telangana, India; 2Department of Microbiology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences (SIMATS), Chennai, India; 3Department of Microbiology, Mallareddy Institute of Medical Sciences, Hyderabad, Telangana, India; 4Faculty of Allied Health Sciences, Chettinad Hospital and Research Institute, Chettinad Academy of Research and Education, Kelambakkam-603103, Tamilnadu, India

 

Email

V Shravani- shravanisunkoju@gmail.com

AS Smiline Girija smilinejames25@gmail.com

Himabindu himabindu2001@yahoo.com

Madhan Krishnan- kmadhan91@gmail.com

Shyamaladevi Babu- shyamdevi06@gmail.com

 

Article Type

Research Article

 

Date

Received March 1, 2023; Revised March 31, 2023; Accepted March 31, 2023, Published March 31, 2023

 

Abstract

Quorum sensing (QS) is one of the key virulence factors in Pseudomonas aeruginosa and causes recalcitrant infections. Multi-drug resistance and biofilm formation seem to be regulated by cell-to-cell communication system through QS. Thus this study is aimed to assess the efficacy of ethanolic leaf extract of Boerhavia diffusa in acting against the QS-regulated virulence traits. Fresh leaves of B. diffusa were dried and the ethanolic crude extract was checked for antimicrobial and anti biofilm effect against P. aeruginosa. The active components and the biological structures were elucidated by GC-MS, HPLC and NMR analysis respectively. Further, computational analyses were also performed to assess the drug ligand interactions based on the docking scores and binding energy. The results suggested that the MIC concentration showed a significant effect in inhibiting the QS network circuit of P. aeruginosa. The docking results showed that leaf had bioactive compounds that exhibit strong binding affinity towards transcriptional activators of the QS circuit in P. aeruginosa, i.e., LasR, as compared to the natural ligands, 3-oxo-C12-HSL and C4-HSL. These results clearly depictthe efficacy of Boerhavia diffusa and its phyto-constituents as promising QS antagonist which can be further applied in the treatment strategies for the diseases caused by P. aeruginosa.

 

Keywords

Pseudomonas aeruginosa, virulence factors, Boerhavia diffusa, molecular docking

 

Citation

Shravani et al. Bioinformation 19(3): 310-318 (2023)

 

Edited by

P Kangueane

 

ISSN

0973-2063

 

Publisher

Biomedical Informatics

 

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.