Title

Serial quantification of CRP and total leukocyte count as a complementary tool in neonatal sepsis

Authors

V. Gomathi¹, G. Chitrasivasankari^1,*, T. Uma¹, M. P. Saravanan² & Kaarthikeyan Gurumoorthy³

Affiliation

¹Institute of Biochemistry, Madras Medical College, RGGGH, Chennai, India; ²Institute of Biochemistry, Stanley Medical College, Chennai;³Saveetha Dental College& Hospital, Saveetha Institute of Medical and Technical Sciences (SIMATS), Chennai – 77, India; *Corresponding author

Email

V. Gomathi– E-mail: gomathivdr@gmail.com

G. Chitrasivasankari - E-mail:drmcvk7896@gmail.com, Phone: +91 9894204838

T.  Uma - E-mail: bhas-2001in@yahoo.com

M.P. Saravanan - E-mail: maduraipandiansaravanan@gmail.com

GurumoorthyKaarthikeyan- E-mail: kaarthikeyang78@gmail.com

Article Type

Research Article

Date

Received September 2, 2022; Revised October 3, 2022; Accepted October 6, 2022, Published October 31, 2022

Early detection and appropriate treatment of newborn sepsis reduce mortality and morbidity. A rapid, inexpensive laboratory approach is needed to assess newborn sepsis, even though blood culture is the gold standard for diagnosis. To compare serial CRP and Total Leukocyte Count (WBC) with blood culture, this study aimed to evaluate the role of newborn sepsis. A total 148 neonates with clinical symptoms of sepsis were included .CRP was measured by quantitative immuno turbidimetric method andotal leukocyte count (WBC) was measured by automated cell counter. CRP1 and WBC1 were measured within 6 hours of clinical symptoms. CRP2 and WBC2 were measured after 48 hours of clinical symptoms. Sensitivity, specificity, PPV, NPV of CRP1 and CRP2,WBC 1and WBC 2 were compared with culture positive and negative sepsis.CRP 2 showed high sensitivity 96% and high NPV95% with significant p value <0.0001. WBC2 has high sensitivity (90.57%) and NPV (91%) with significant p value <0.0001. CRP 1 has sensitivity 83%and NPV 82.3%, with p value < 0.001.WBC1 has lowest sensitivity (62.2%) and NPV (71.4%) compared to all other parameters. Serial CRP and WBC measurements are useful in the diagnosis of neonatal sepsis. Measurement of CRP and Total Leukocyte Count (WBC) after 48 hours of clinical symptoms were considered promptly for diagnose neonatal sepsis.

Keywords

Neonatal sepsis, C-reactive protein, total leukocyte Count

Citation

Gomathi et al. Bioinformation 18(10): 920-924 (2022)

Edited by

P Kangueane

ISSN

0973-2063

Publisher

Biomedical Informatics

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.