Title
PCR primer design for mitochondrial cox-1 gene from Clinostomum complanatum towards diagnosis
Authors
Monica Misra1, Bhumika Chauhan1, Km Deepika2, A. Madhuri3 & Bindu Sharma4,*
Affiliation
1Department of Zoology, Acharya Narendra Dev College, University of Delhi, India; 2Laboratory of Molecular Parasitology, Department of Zoology, Chaudhary Charan Singh University, Meerut, India; 3Government Degree College for Women, Begumpet, Hyderabad, India; 4Laboratory of Molecular Parasitology, Department of Zoology, Chaudhary Charan Singh University, Meerut, India
*Corresponding author
Bindu Sharma – E-mail: dr.bindusharmazoology@gmail.com
Article Type
Research Article
Date
Received September 2, 2022; Revised September 30, 2022; Accepted September 30, 2022, Published September 30, 2022
Abstract
Metacercariae of Clinostomum Leidy, 1856 are frequently encountered in freshwater fish. Clinostomum complanatum is a digenetic zoonotic parasite harbouring the intestine and body cavity of the fishes. 19 human incidences of Clinostomum complanatum infection have been reported to cause pharyngitis and lacramalitis from Japan, Thailand and Korea. Hence, adequate yet effective diagnosis is an issue. Designing primers used in the amplification of genes with adequate specificity and efficiency is of help in diagnosis. Hence, we describe primer design for cox-1 gene from the helminth parasite, Clinostomum complanatum parasitizing the intestine of fish Channastriata (Snakehead murrel). Thus, these designed primers set will be of further use in the wet lab for amplification of concerned gene or DNA fragment.
Keywords
Clinostomum complanatum, zoonotic, primer, design, cox-1, gene, DNA
Citation
Misra et al. Bioinformation 18(9): 831-833 (2022)
Edited by
P Kangueane
ISSN
0973-2063
Publisher
License
This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.
