Title
Effect of Carica papaya on beta catenin and Wnt mRNA expression in human colon cancer (HT-29) cells in vitro
Authors
Mohamed Rifaath, Preetha Santhakumar & J. Selvaraj
Affiliation
Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, 162, Poonamallee High Road, Chennai - 600077, Tamilnadu, India; Phone: +91 8608566435
Mohamed Rifaath - E-mail: Rifaath.a@icloud.com
S. Preetha - E-mail: preethas.sdc@saveetha.com
J. Selvaraj - E-mail: selvarajj.sdc@saveetha.com
Article Type
Research Article
Date
Received January 21, 2022; Revised March 31, 2022; Accepted March 31, 2022, Published March 31, 2022
Abstract
Colon cancer is the third most frequent cancer in humans. Carica papaya leaves are vegetable foods consumed by most people around the world; it has potential as an anticancer. Therefore it is of interest to investigate the effect of Carica papaya on beta catenin and Wnt mRNA expression in human colon cancer (HT-29) cells in vitro. Human Colon cancer cell line (HT-29) was purchased from the National Centre for Cell Sciences, Pune, India. Cell viability test was done by MTT assay. Gene expression analysis was done by Real Time-PCR. The obtained data were analyzed statistically by one-way analysis of variance and Duncan's multiple range test with Graph Pad Prism version 5 to analyze the significance of individual variations among the control and experimental groups. The significance was considered at p<0.05 level in Duncan's test. Carica papaya caused a marked increase in cell death in a dose dependent manner. At the end of 48 hours, maximum inhibition was at 300 and 400 μg/ml. Carica papaya has significantly reduced the mRNA expression of Wnt and beta catenin (p<0.05). Data showed that Carica papaya leaf extract has anticancer activity on Colon cancer cell lines (HT-29).
Keywords
Carica papaya, beta catenin, Wnt, mRNA expression, human colon cancer (HT-29) cells, in vitro
Citation
Rifaath et al. Bioinformation 18(3): 289-292 (2022)
Edited by
P Kangueane
ISSN
0973-2063
Publisher
License
This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.
