Title

Intrinsically disordered regions in the rodent hepevirus proteome

Authors

Zoya Shafat¹, Anwar Ahmed², Mohammad K. Parvez³, Asimul Islam¹, Shama Parveen^1,*

Affiliation

¹Centre for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, New Delhi, India; ²Centre of Excellence in Biotechnology Research, College of Science, King Saud University, Riyadh, Saudi Arabia; ³Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia; Corresponding author* - Shama Parveen

Email

Shama Parveen - sparveen2@jmi.ac.in; Zoya Shafat - zoya179695@st.jmi.ac.in; Anwar Ahmed - anahmed@ksu.edu.sa; Asimul Islam aislam@jmi.ac.in; Mohammad K Parvez - mohkhalid@ksu.edu.sa

Article Type

Research Article

Date

Received December 8, 2021; Revised January 3, 2022; Accepted January 3, 2022, Published February 28, 2022

Hepatitis E virus (HEV) is the causative agent of Hepatitis E infections across the world. Intrinsically disordered protein regions (IDPRs) or intrinsically disordered proteins (IDPs) are regions or proteins that are characterized by lack of definite structure. These IDPRs or IDPs play significant roles in a wide range of biological processes, such as cell cycle regulation, control of signaling pathways, etc. IDPR/IDP in proteins is associated with the virus’s pathogenicity and infectivity. The prevalence of IDPR/IDP in rat HEV proteome remains undetermined. Hence, we examined the unstructured/disordered regions of the open reading frame (ORF) encoded proteins of rat HEV by analyzing the prevalence of intrinsic disorder. The intrinsic disorder propensity analysis showed that the different ORF proteins consisted of varying fraction of intrinsic disorder. The protein ORF3 was identified with maximum propensity for intrinsic disorder while the ORF6 protein had the least fraction of intrinsic disorder. The analysis revealed ORF6 as a structured protein (ORDP); ORF1 and ORF4 as moderately disordered proteins (IDPRs); and ORF3 and ORF5 as highly disordered proteins (IDPs). The protein ORF2 was found to be moderately as well as highly disordered using different predictors, thus, was categorized into both IDPR and IDP. Such disordered regions have important roles in pathogenesis and replication of viruses.

Keywords

Rat hepevirus; open reading frame (ORF); intrinsic disorder; structured protein; moderately disordered protein; highly disordered protein; ordered protein; intrinsically disordered protein (IDP); intrinsically disordered protein region (IDPR).

Citation

Shafat et al. Bioinformation 18(2): 111-118 (2022)

Edited by

P Kangueane

ISSN

0973-2063

Publisher

Biomedical Informatics

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.