Title

MTT assay based inhibition analysis of A2780 cells proliferation using Mollugo nudicaulis Lam. extract with CXCR4 and HER2 expression

Authors

Chella Perumal Palanisamy^1,*, Shazia Fathima JH², Ramya Sekar³, Ponnulakshmi Rajagopal⁴ & Selvaraj Jeyaraman^5*

Affiliation

¹State Key Laboratory of Biobased Material and Green Papermaking, School of Food Science and Engineering, Qilu University of Technology, Shandong Academy of Science, Jinan 250353, China;  ²Department of Oral and Maxillofacial Pathology, Ragas Dental College and Hospitals, Chennai, India; ³Department Oral Pathology, Meenakshi Ammal Dental College, Chennai-600 095, India; ⁴Central Research Laboratory, Meenakshi Academy of Higher Education and Research (Deemed to be University), Chennai-600 078, India; ⁵Department of Biochemistry, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai - 600 077, India. ^*Corresponding authors;

Email

Dr. Selvaraj Jeyaraman - Email:jselvaendo@gmail.com; perumalbioinfo@gmail.com;

Article Type

Research Article

Date

Received May 5, 2021; Revised July 19, 2021; Accepted July 19, 2021, Published July 31, 2021

It is of interest to document the inhibition of A2780 cell proliferation using Mollugo nudicaulis Lam. (M. nudicaulis) extract by MTT assay and by monitoring the CXCR4 and HER2 expression through RT-PCR analysis. Results shown that the n-hexane extract of M. nudicaulis have anticancer activity IC50 values of 32.46±0.92 Ĵg/mL on A2780 cell lines. It is further found that the CXCR4 and HER2
mRNA and protein expression were significantly reduced in M. nudicaulis treated A2780 cell lines. Thus, the n-hexane extract of M. nudicaulis is a natural source of bioactive compounds as potential anticancer agents.

Keywords

M. nudicaulis; n-hexane extract; CXCR4 and HER2 protein expression, Metastatic signalling, Anti-ovarian cancer activity

Citation

Palanisamy et al. Bioinformation 17(7): 705-709 (2021)

Edited by

P Kangueane

ISSN

0973-2063

Publisher

Biomedical Informatics

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.