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In vitro cytotoxicity analysis of Zizyphus spina-christi stem bark extract on human cancer cell lines



Saravanan Rajendrasozhan1*, Essam N. Ads2, Amr S. Abouzied3,4 & Jamal R. Humaidi1



1Department of Chemistry, College of Sciences, University of Ha!il, Ha!il 55476, Kingdom of Saudi Arabia; 2Faculty of Science, Zagazig University, El Mansoura 35522, Egypt; 3Department of Pharmaceutical Chemistry, College of Pharmacy, University of Ha!il, Ha!il 55476, Kingdom of Saudi Arabia; 4Department of Pharmaceutical Chemistry, National Organization for Drug Control and Research, Giza 35521, Egypt.



Saravanan Rajendrasozhan - Email: s.rajendrasozhan@uoh.edu.sa; Essam N. Ads - Email: essam.adss@yahoo.com; Amr S. Abouzied - Email: as.ibrahim@uoh.edu.sa; Jamal R. Humaidi - Email: j.humaidi@uoh.edu.sa


Article Type

Research Article



Received April 29, 2021; Revised May 29, 2021; Accepted May 29, 2021, Published May 31, 2021



Zizyphus spina-christi (Rhamnaceae family) is an edible plant used in folk medicine. Therefore, it is of interest to report the cytotoxic effects of Z. spina-christi bark crude extract on human cell lines. Crude ethanol extract of Z. spina-christi bark was fractionated with increasing polarity (diethyl ether, chloroform, ethyl acetate and butanol fractions). The fractions were examined for their cytotoxicity against human colon cancer (HCT-116 and CACO-2), cervical cancer (HeLa and HEp-2), lung carcinoma (A-549), hepatocellular carcinoma (HepG-2), breast cancer (MCF-7) and prostate cancer (PC-3) cell lines using viability assay. Diethyl ether fraction of Z. spina-christi showed the highest cytotoxic effects among the four extracts of Z. spina-christi. The IC50 of diethyl ether fraction was 7.14, 11.2, 11.6, 15.4, 39.8, 42.2, 84.2 and 153.8 Ĵg/ml on HepG-2, A-549, CACO-2, HCT-116, MCF-7, PC-3, HeLa, and HEp-2 cell lines, respectively. Data shows that the diethyl
ether fraction of Z. spina-christi showed effective cytotoxic effects in colon, lung and hepatocellular cancer cell lines.



Anticancer; cytotoxic; phytochemicals; natural remedies, Zizyphus spina-christi



Rajendrasozhan et al. Bioinformation 17(5): 583-592 (2021)


Edited by

P Kangueane






Biomedical Informatics



This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.