Title
Polymorphisms in vitamin D receptor, toll-like receptor 2 and Toll-Like receptor 4 genes links with Dengue susceptibility
Authors
Arvind Kumar Singh1, Shantanu Prakash1, RK Garg2, Parul Jain1, Rashmi Kumar3 & Amita Jain1,*
Affiliation
1Department of Microbiology King George’s Medical University, Lucknow, UP, India; 2Department of Neurology King George’s Medical University, Lucknow, UP, India; 3Department of Paediatrics King George’s Medical University, Lucknow, UP, India; Amita Jain - Corresponding Author*
Email- amita602002@yahoo.com; Mobile No: 9415023928
Article Type
Research Article
Date
Received February 28, 2021; Revised March 31, 2021; Accepted April 10, 2021, Published April 30, 2021
Abstract
Host genetic factors are known to determine disease susceptibility in dengue virus infection. Therefore, in this study association of gene polymorphisms of Vitamin D Receptor [rs731236 (Taq) and rs7975232(Apa1)], Toll-like receptor 2 [rs5743708(Arg735Gln) and rs5743704(Pro631His)] and Toll-like receptor 4 [rs4986790A/G(Asp299Gly13843) and rs4986791 C/T(Thr399Ile)] were studied in cases with dengue as compared to controls. Total 98 cases of confirmed dengue virus infection and 98 age, sex and geographically matched healthy controls were enrolled and their genetic polymorphisms for the above mentioned regions were studied by Sanger sequencing. Mutant genotypes CC of VDR rs731236(Taq1) [(OR 3.808, p value =0.02, CI 1.160-12.498)], GG of VDR rs7975232 (Apa1) [(OR 3.485, p value =0.02, CI 1.162-10.45)] and heterozygous genotypes of TLR4 rs4986790 A/G Asp299Gly [OR 2.40, p value= 0.02, CI 1.12-5.14], TLR4 rs4986791 C/T Thr399Ile [OR 2.09, p value=0.02, CI 1.12-5.14] were found to be significantly more in cases with dengue virus infection as compared to the controls. Also, at these positions mutant alleles were observed in significantly higher number of cases than controls. The values for C allele at VDR rs731236(Taq1) were OR 1.86, p value 0.009, CI 1.162-3.001; for allele G at rs7975232(Apa1) were OR 2.71, p value 0.006, CI 1.196-2.98 for allele G at TLR4s rs4986790 A/G Asp299Gly were OR 2.35, p value 0.009, CI 1.23-4.50 and for allele T at rs4986791 C/T Thr399Ile were OR 2.36, p value=0.006, CI 1.28-4.38. VDR and TLR4 but not TLR2 gene polymorphisms were found to be associated with dengue susceptibility in Indian population.
Keywords
Innate Immunity, Dengue virus Infection, SNP, Toll like Receptor, TLR 2, TLR4, Vitamin D receptor (VDR).
Citation
Singh et al. Bioinformation 17(4): 506-513 (2021)
Edited by
P Kangueane
ISSN
0973-2063
Publisher
License
This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.
