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¹Senior Lecturer, Faculty of Medicine, Bioscience and Nursing, School of Bioscience, Mahsa University, Saujana Putra Campus, Jalan SP2, Bandar Saujana Putra, 42610, Jenjarom, Selangor, Malaysia; ²Research Scholar, Muthayammal College of Arts & Science (A Unit of VANETRA Group), Rasipuram, 637408, Namakkal, Tamilnadu, India; ³Department of Zoology & Biotechnology, Muthayammal College of Arts & Science (A Unit of VANETRA Group), Rasipuram, 637408, Namakkal, Tamilnadu, India; ⁴Department of Biochemistry, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai - 600 077; ⁵Adjunct Associate Professor, Muthayammal Centre for Advanced Research (MCAR), Muthayammal College of Arts & Science (A Unit of VANETRA Group), Rasipuram, 637408, Namakkal, Tamilnadu, India;

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The immature lymphoid cells with chromosomal structural and numerical abnormalities cause the acute lymphoblastic leukemia (ALL). This hematologic disorder constitutes about 25% of cancer prognosis among children and adolescents. D-Carvone, a monocyclic monoterpene obtained from the essential oils extracted from plants is reported to possess the various biological activities. The present study was aimed to investigate the anticancer potential of D-Carvone against the human leukemic Molt-4 cells. The cytotoxicity of DCarvone was analyzed by MTT assay. The level of lipid peroxidation and antioxidants were determined. The intracellular ROS, MMP and apoptosis were demonstrated by fluorescent staining techniques. The MTT assay revealed that the D-Carvone treatment suppressed the viability of Molt-4 cells and the IC50 was determined at 20 μM/ml. The D-Carvone treatment was increased the oxidative stress and reduced the level of antioxidants in the Molt-4 cell lines. The increased intracellular ROS, apoptotic cell death, and diminished MMP was
noted in the D-Carvone treatment. In the Molt-4 cells, D-carvone induced the apoptosis in a time and dose dependent manner by the activation of caspases-8, -9 and -3. Thus, data provide insights for the clinical application of D-Carvone in the treatment of blood cancer Molt-4 cells. Our study suggests the therapeutic potential D-Carvone for the treatment of leukemia in future.

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Edited by

P Kangueane

ISSN

0973-2063

Publisher

Biomedical Informatics

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This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.