Title

Regulation of pro and anti-inflammatory signaling molecules in effect of Withania Somnifera root extract treated sleep deprivation induced Wistar rats

Authors

K. Suganya^1,2, E. Kayalvizhi³, K. Senthil Kumar³, R. Muthulakshmi², R. Yuvaraj4

Affiliation

¹Sri Ramachandra Medical College, Porur, Chennai, Tamil Nadu, India; ²Meenakhshi Academy of Higher Education and Research, Enathur, Kancheepuram, Tamil Nadu, India; ³Madha Medical College, Kundrathur, Chennai, Tamilnadu, India; ⁴Bharath University, Selaiyur, Chennai, Tamilnadu, India

Email

Dr. E. Kayalvizhi - Email: kayalgkbs@sriramachandra.edu.in; Mrs. K. Suganya - Email: k.suganya@sriramachandra.edu.in; *Corresponding author

Article Type

Research Article

Date

Received September 9, 2020; Revised September 28, 2020; Accepted September 28, 2020; Published November 30, 2020

Sleep plays an imperative role in maintaining good health. Sleep along with circadian cycle wields strong regulatory control over immunity. Sleep deprivation (SD) is a threat to health developing several immunological disorders. The medicinal plant Withania somnifera (WS) root extract is widely used for its immuno-modulatory properties. Therefore, it is of interest to assess the effect of WS root extract on pro and anti-inflammatory signalling in SD rats. 24 male Wistar rats (120-150g) were divided into 4 groups with 6 animals in each. The groups were divided such that Group I - cage control, Group II - large platform control, Group III - sleep deprived & Group IV – WS treated SD rats. RT-PCR based mRNA expression analysis of pro inflammatory (IL-1β, IL-6, MCP-1, TNF-α) and anti-inflammatory marker (IL-10) in the cortex of control and SD rats were completed. Concurrent protein expression analysis was completed using western blot. Data was analyzed using one-way ANOVA and Duncan’s multiple range test in SPSS software version 20. Data showed elevation of pro-inflammatory markers and depression of IL-10. Thus, WS down regulated the pro-inflammatory and up-regulated the anti-inflammatory molecules, which can be further considered towards the treatment of sleep deprivation induced inflammatory diseases.

Keywords

Sleep Deprivation (SD), Withania Somnifera (WS), modified multiple platform method, pro-inflammatory markers, anti-inflammatory marker.

Citation

Suganya et al. Bioinformation 16(11): 856-862 (2020)

Edited by

P Kangueane

ISSN

0973-2063

Publisher

Biomedical Informatics

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.