Title

Link between PON 1 gene mutation and recurrent miscarriage among women exposed to pesticides in North India is insignificant

Authors

Akancha Pandey¹, Shyam Pyari Jaiswar^1,*, Sujata Deo¹, Pushplata Sankhwar¹, Mohammad Kaleem
Ahmad², Shriya Pant³

Affiliation

¹Department of Obstetrics and Gynecology, King Georg’s Medical University, Lucknow, Uttar Pradesh, India; ²Department of Biochemistry, King Georg’s Medical University, Lucknow, Uttar Pradesh, India; ³Department of Urology, King Georg’s Medical University, Lucknow, Uttar Pradesh, India

Email

Akancha Pandey – akancha0506@gmail.com; E-mail: Shyam Pyari Jaiswar – E-mail:
spjaiswar@yahoo.com*Corresponding Author

Article Type

Research Article

Date

Submitted on August 31, 2020; Revision September 14, 2020; Accepted September 14, 2020; Published October 31, 2020

Recurrent miscarriage is a loss of disconcerting disorder characterized by RPL (recurrent pregnancy loss) of pregnancy, affecting around 1-2% of couples trying to conceive. Exposure to pesticide affects spontaneous abortion, and infertility in women. Placental oxidative stress is often linked to miscarriage. Therefore, it is of interest to link PON1 (Q1922R) polymorphism with recurrent pregnancy loss. We selected 200 subjects in which 100 patients with RPL having consecutive 2 or more miscarriages and 100 healthy controls from the northern India for this study. Blood samples were collected for DNA isolation and further assessment. Genotyping of the Q1922R polymorphism was completed using the RFLP markers. The digested PCR product size was 99 bp (control). The heterozygous fragments were found to be 66 and 33 bp homozygous mutants. It was observed that allele frequency homozygous (TT) was more prevalent among control than the case groups among the healthy north-Indian population. However, heterozygous group (Tt) was more in cases compared to control groups as well as homozygous mutant was observed high in control in than case (CI-0.3 to 1.3).

Keywords

Recurrent pregnancy loss, Polymorphism, Pesticides, PON1, RFLP.

Citation

Pandey et al. Bioinformation 16(10): 779-785 (2020)

Edited by

P Kangueane

ISSN

0973-2063

Publisher

Biomedical Informatics

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.