Title

Anti-inflammatory, anti-oxidant effect and cytotoxicity of Ocimum sanctum intra oral gel for combating periodontal diseases

Authors

Jaiganesh Ramamurthy^1,* & Nadathur Duraisamy Jayakumar²

Affiliation

¹Department of Periodontics, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Chennai, India; ²Department of Periodontics, Saveetha Dental College and Hospitals, Saveetha Institute of Medical
and Technical Sciences (SIMATS), Saveetha University, Chennai, India

Email

Jaiganesh Ramamurthy, Email - jaiganeshr@saveetha.com, Corresponding author*

Article Type

Research Article

Date

Received October 7, 2020; Revised October 27, 2020; Accepted October 27, 2020; Published December 31, 2020

It is of interest to evaluate the anti-inflammatory, anti-oxidant effect and cytotoxicity of Ocimum sanctum (an Indian herb, Thulsi) intra oral gel in combating periodontal diseases. Hence, 2% of O. sanctum gel was prepared with Carbopol940 soaked in purified water containing
0.2% w/v sodium benzoate overnight. Hydroxy proplyl methyl cellulose (HPMC) solution was mixed with propylene glycol using using tissue homogenizer. Anti-oxidant effect was analyzed using DPPH radical assay and anti-inflammatory effect was assessed using the
inhibition of albumin denaturation assay. Ocimum sanctum gel with various dilutions from10 micro litres to 50 micro litres showed exponential increase in percentage of inhibition from 60.9 to 72.2 exhibiting antioxidant activity. The anti-inflammatory effect of Ocimum
sanctum gel showed comparatively equivalent effect with standard diclofenac gel with values ranging from 76.6 for 50 micro liters of Ocimum sanctum gel and 89.6 for standard gel at 50 micro liters. Ocimum sanctum showed less toxicity towards brine shrimp nauplii. Thus
we show that Ocimum sanctum gel showed potent anti-oxidant and anti-inflammatory effect and less toxic to brine shrimp nauplii as a promising agent for the treatment of periodontal diseases.

Keywords

Ocimum sanctum, periodontal diseases, anti-oxidant effect, anti-inflammatory effect, cytotoxic effect.

Citation

Ramamurthy & Jayakumar, Bioinformation 16(12): 1026-1032 (2020)

Edited by

P Kangueane

ISSN

0973-2063

Publisher

Biomedical Informatics

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.