Title

Genetic association of MBL-2 gene polymorphisms with Filarial chyluria

Authors

¹Shriya Pant, ¹Apul Goe^l*, ¹Pravin Kumar Gangwar, ²Jyotsna Agarwal, ³Arvind Kumar Singh, ¹Satya
Narayan Sankhwar; ³Prashant Gupta

Affiliation

¹Department of Urology, King George's Medical University, Lucknow, UP, India; ²Department of Microbiology, Dr. Ram Manohar Lohia Institute of Medical Sciences, India; ³Department of Microbiology, King George's Medical University, Lucknow, UP, India

Email

Apul Goel – Email:drapul.goel@gmail.com; *Corresponding author

Article Type

Research Article

Date

Received November 14, 2019; Revised November 28, 2019; Accepted December 7, 2019; Published December 10, 2019

Lymphatic filariasis has become a significant public health issue in North India. The association of polymorphisms in MBL2 gene with filarial chyluria (FC) is evaluated in the North Indian patients for the first time. Hence, a tertiary care hospital based case-control study was conducted in north India where FC is endemic. Therefore, 186 confirmed patients of FC as cases and 210 age-, sex- and residence-matched subjects as controls were enrolled for the study. Filarial etiology was confirmed using diethylcarbamazine (DEC)-provocation test, immune chromatographic test and IgG/IgM antibody test. MBL2 gene polymorphisms at codon 54 and -221 promoter region were genotyped by
PCR followed by RFLP. Wild-type, heterozygous and homozygous mutant frequencies of MBL2 genotype at the codon 54 were 57.5%, 32.8% and 9.7% in the case group and 62.9%, 30.5% and 6.7%, in controls, respectively. The same at the -221 position were 51.1%, 44.1% and 4.8% in FC patients and 44.3%, 40.0% and 15.7% in controls, respectively. Thus, results no significant association between MBL2 polymorphism at codon 54 and FC. However, polymorphism at the -221 promoter region is linked with FC with a significant odd-ratio of 0.27 (confidence interval at 95% was 0.12-0.59; p<0.001). This preliminary finding is intriguing for further confirmation using a larger study with more patients.

Keywords

Mannose-binding lectin; lymphatic filariasis; chyluria.

Citation

Pant et al. Bioinformation 15(11): 806-811 (2019)

Edited by

P Kangueane

ISSN

0973-2063

Publisher

Biomedical Informatics

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.