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Phyllanthus amarus protects against spatial memory impairment induced by lipopolysaccharide in mice



Akilandeshwari Alagan1, Ibrahim Jantan2, Endang Kumolosasi1 & Norazrina Azmi1*



1Drug and Herbal Research Centre, Faculty of Pharmacy, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia; 2School of Pharmacy-SRI, Faculty of Health & Medical Sciences, Taylorís University, Lakeside Campus, Jalan Taylor's, 47500 Subang Jaya, Selangor, Malaysia.




Article Type

Research Article



Received August 9, 2019; Accepted August 14, 2019; Published August 31, 2019



Phyllanthus amarus Schumach. and Thonn. is a wide spread medicinal herb with various traditional uses. It is well documented for its antioxidant, anti-inflammatory, and hepatoprotective activities. Therefore, it is of interest to evaluate the 80% ethanol extract of Phyllanthus amarus (PA) on spatial memory using the 8-radial arm maze (8-RAM) in mice after induction of neuroinflammation by lipopolysaccharide (LPS) in a 14- and 28-days treatment study. LC-MS/MS was performed to profile the chemical composition in PA extract. Mice were treated orally with 5% v/v tween 20, PA extract (100, 200 and 400 mg/kg), or ibuprofen (IBF 40 mg/kg) for 14 and 28 days. All groups
were challenged with LPS (1 mg/kg) via intraperitoneal (i.p.) injection a day prior to the 8-RAM task except for the negative control group which received an i.p. injection of saline. Data obtained were analyzed with one-way ANOVA followed by post hoc Dunnett's test (comparison of all groups against vehicle control). Analysis of LC-MS/MS data revealed the presence of 16 compounds in the PA extract. Administration of PA extract at 200 and 400 mg/kg for 14 and 28 days significantly (*P<0.05) decreased the working and reference memory errors against LPS-induced spatial memory impairment. The observed protective action is possibly due to the putative antineuroinflammatory
effects of PA. In conclusion, PA extract possess neuroprotective effects against spatial memory impairment mediated by LPS.



Phyllanthus amarus, spatial memory, neuroprotection, neuroinflammation, lipopolysaccharide.



Alagan et al. Bioinformation 15(8): 535-541 (2019)


Edited by

P Kangueane






Biomedical Informatics


License This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.