Title

Oncogenic Human Papillomavirus involvement in epithelial ovarian carcinoma among women in Morocco

Authors

Rahma Ait Hammou¹, Mustapha Benhessou^1,2,3, Amal Bouziyane², Najwa Hassou¹, Mohammed Nabil Benhchekroun¹, Hlima Bessi¹, Moulay Mustapha Ennaji^1*

Affiliation

¹Laboratory of Virology, Microbiology, Quality and Biotechnologies/Eco-toxicology and biodiversity, faculty of Sciences and Techniques, Mohammedia, University Hassan II of Casablanca, BP: 146 Mohammedia 20650, Morocco; ²Université Mohamed VI des Sciences de la santé of Casablanca. Morocco; 3Faculty of Medicine and Pharmacy of Casablanca, University Hassan II of Casablanca, Morocco

Email

Moulay Mustapha Ennaji - Email: m.ennaji2018@gmail.com; *Corresponding author

Article Type

Research Article

Date

Received December 20, 2018; Accepted January 7, 2019; Published February 3, 2019

Epithelial Ovarian cancer (EOC) although rare is the most lethal gynecological cancer in women worldwide. Despite its high prevalence few studies have been performed to evaluate the prevalence and determinants of HPV infection worldwide. The aim of the present study was to investigate the presence of HPV-DNA in Moroccan patients with EOC using PCR among women in Casablanca, and to examine the prevalence of some HPV genotypes in Moroccan population. We performed a study of HPV detection on Fresh biopsies of 70 epithelial ovarian cancer patients. PCR was realized using the MY09/11 and GP5+/6+ primers. Genotyping of HPV was performed by PCR type specific for HPV 6, 11, 16, 18, 31, and 33.Data was statistically analyzed using SPSS software. Hence, the mean age was 48.9 years (range, 21–76 years). Serous adeno carcinoma (75.71%) and stage III of the disease represent the majority of cases. eight patients were HPV positive (11.42%).Results of HPV genotyping revealed predominance of two genotypes: HPV 16 (87.5%) and HPV 31(12.5).No co-infection identified. Approximately 75% of positive cases had a serous cystadeno carcinoma and more than 62,5% had FIGO advanced stage (III or IV). Our study showed that high-risk HPV infection could play a major role among patients with EOC in Morocco.

Keywords

Oncogenic Human Papillomavirus, fresh biopsies, epithelial ovarian carcinoma, viral carcinogenesis, Genotyping, PCR typespecific.

Citation

Ait Hammou et al. Bioinformation 15(1): 55-60 (2019)

Edited by

P Kangueane

ISSN

0973-2063

Publisher

Biomedical Informatics

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.