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Title

Molecular characterization of leaf spot fungi using internal transcribed spacer (ITS) based phylogenetic inference

 

Authors

Bose Chitrakani1, Senthuran Sureshkumar1, Pandy Rajapriya2 & Mohan Pandi1,*

Affiliation

1Department of Molecular Microbiology, School of Biotechnology, Madurai Kamaraj University, Madurai- 625 021, Tamil Nadu, India; 2Department of zoology, M.S.S, Wakf Board college, Madurai - 625 020, Tamil Nadu, India
 

Email

 Mohan Pandi - E-mail: an_pandi@rediffmail.com; Tel: +91-452-2458208; Fax: +91-452-2459105. *Corresponding author

 

Article Type

Research Article

 

Date

Received January 8, 2019; Accepted January 18, 2019; Published February 3, 2019

 

Abstract

The plant pathogenic leaf spot fungi cause loss in crop yield. Leaf spot diseases are caused by fungi and other pathogens such as a virus, bacteria, and nematodes play a secondary role. Therefore, it is of interest to study internal transcribed spacer (ITS) sequence from the plant pathogenic fungi. Hence, we collected nineteen different isolates at Madurai Kamaraj University, Tamilnadu, India for this study. We report nineteen positive isolates identified with species-level characterization using ITS sequence supported with a phylogenetic tree and corresponding secondary structure analysis.

 

Keywords

Leaf spot fungi, ITS, Secondary structure, phylogenetic tree.

 

Citation

Chitrakani et al. Bioinformation 15(1): 46-54 (2019)

 

Edited by

P Kangueane

 

ISSN

0973-2063

 

Publisher

Biomedical Informatics

 

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.