BACK TO CONTENTS   |    PDF   |   


Phytochemical derivatives targeting fliJ flagellar protein from Escherichia coli


C. R. Hemalatha1*, P. A. Abhinand1, Maithreyi Iyer1, Benedict C. Paul1, Anupma Jyoti Kindo1, Thyagarajan Ravinder2, P. Dhanapalan3



1Sri Ramachandra Institute of Higher Education and Research, Porur, Chennai 600116; 2Govt. Kilpauk Medical College and Hospital, Chennai 600010; 3Madras Veterinary College, Chennai 600007.



C. R. Hemalatha


Article Type




Received September 25, 2018; Revised October 3, 2018; Accepted October 5, 2018; Published October 30, 2018



Approximately 50 per cent of nosocomial infections are caused by the use of indwelling medical devices. The surfaces of devices are ideal sites of attachment for bacterial cells and an increase in biofilm formation. Biofilms have been a constant concern due to their complex extracellular matrix (ECM) resulting in multiple drug resistance. E. coli is known to associate with biofilms. Therefore it is of interest to identify the proteins associated to biofilm formation in Escherichia coli through literature survey, investigate their protein-protein interactions and identify indispensible proteins of biofilm formation. These proteins were further analyzed and fliJ was identified as the target, based on betweenness, centrality and radiality. 87 phytochemicals were found to be associated with the microbe in question and were docked with the target using Molegro Virtual Docker (MVD) 5.0. The results showed that geranyl pyrophosphate, ferulic acid 4-o-b-d-glucuronide, 5-8'-dehydrodiferulic acid and geranyl diphosphate showed maximum activity. A combinatorial library of 96 models was generated using the four phytochemicals binding with fliJ.



Biofilms, E. coli, fliJ, flagellar protein, phytochemical derivatives



Hemalatha et al. Bioinformation 14(9): 465-470 (2018)


Edited by

P Kangueane






Biomedical Informatics



This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.