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Title

Phytochemical derivatives targeting fliJ flagellar protein from Escherichia coli

Authors

C. R. Hemalatha1*, P. A. Abhinand1, Maithreyi Iyer1, Benedict C. Paul1, Anupma Jyoti Kindo1, Thyagarajan Ravinder2, P. Dhanapalan3

 

Affiliation

1Sri Ramachandra Institute of Higher Education and Research, Porur, Chennai 600116; 2Govt. Kilpauk Medical College and Hospital, Chennai 600010; 3Madras Veterinary College, Chennai 600007.

 

Email

C. R. Hemalatha hemalatha@sriramachandra.edu.in

 

Article Type

Hypothesis

 

Date

Received September 25, 2018; Revised October 3, 2018; Accepted October 5, 2018; Published October 30, 2018

 

Abstract

Approximately 50 per cent of nosocomial infections are caused by the use of indwelling medical devices. The surfaces of devices are ideal sites of attachment for bacterial cells and an increase in biofilm formation. Biofilms have been a constant concern due to their complex extracellular matrix (ECM) resulting in multiple drug resistance. E. coli is known to associate with biofilms. Therefore it is of interest to identify the proteins associated to biofilm formation in Escherichia coli through literature survey, investigate their protein-protein interactions and identify indispensible proteins of biofilm formation. These proteins were further analyzed and fliJ was identified as the target, based on betweenness, centrality and radiality. 87 phytochemicals were found to be associated with the microbe in question and were docked with the target using Molegro Virtual Docker (MVD) 5.0. The results showed that geranyl pyrophosphate, ferulic acid 4-o-b-d-glucuronide, 5-8'-dehydrodiferulic acid and geranyl diphosphate showed maximum activity. A combinatorial library of 96 models was generated using the four phytochemicals binding with fliJ.

 

Keywords

Biofilms, E. coli, fliJ, flagellar protein, phytochemical derivatives

 

Citation

Hemalatha et al. Bioinformation 14(9): 465-470 (2018)

 

Edited by

P Kangueane

 

ISSN

0973-2063

 

Publisher

Biomedical Informatics

 

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.