Title

Screening, isolation and characterization of biosurfactant producing Bacillus subtilis strain ANSKLAB03

Authors

Anuraj Nayarisseri^1,2, Poonam Singh³, Sanjeev Kumar Singh^1*

Affiliation

¹Computer Aided Drug Designing and Molecular Modeling Lab, Department of Bioinformatics, Alagappa University, Karaikudi-630 003, Tamil Nadu, India;

²In silico Research Laboratory, Eminent Biosciences, Indore – 452 010, Madhya Pradesh, India; ³Corrosion & Materials Protection Division-/C.S.I.R - Central Electrochemical Research Institute, Karaikudi 6300 06, India;

Email

skysanjeev@gmail.com

Article Type

Hypothesis

Date

Received June 20, 2018; Revised June 25, 2018; Accepted June 27, 2018; Published June 30, 2018

Biosurfactants are surface-active compounds produced by a wide range of microorganisms. They have both hydrophobic and hydrophilic domains and can decrease the surface tension and the interfacial tension of growth medium. Biosurfactants have different chemical structures like-lipopeptides, glycolipids, neutral lipids and fatty acids. They are biodegradable non-toxic biomolecules that show strong emulsification of hydrophobic compounds. They have the ability to form stable emulsions. The low water-solubility of these compounds restricts their availability to microorganisms. Surfactants secreted by microbes enhance the bioavailability of such hydrophobic compounds for bioremediation. Therefore, biosurfactant-enhanced solubility of pollutants has prospective applications in bioremediation. Biosurfactants are useful in a variety of industrial processes, and are also of vital importance to the microbes in adhesion, emulsification, and bioavailability, desorption and defense strategy. Therefore, it is of interest to identify biosurfuctant producing strain of bacteria from brackish water. The microbial samples were isolated from the Chilika Lake, odisha, India and were tested for its biosurfactant property by various biochemical methods. 16S rRNA was sequenced using Sanger dideoxy sequencing method to characterize the biosurfuctant producing strain. The new Bacillus subtilis strain ANSKLAB03 isolated from 40 samples was deposited in GenBank with Accession number KU523257.

Keywords

Biosurfuctant, bacteria, Bacillus subtilis, 16S rRNA, sequence

Citation

Nayarisseri et al. Bioinformation 14(6): 304-314 (2018)

Edited by

P Kangueane

ISSN

0973-2063

Publisher

Biomedical Informatics

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.