Title

Ymr210wp leads to the accumulation of phospholipids and steryl esters in yeast

Authors

Naresh Kumar Manda, Venkata Bala Sai Chaitanya Thunuguntla, Chandrasekhar Bokka, Bondili
Jayakumar Singh*

Affiliation

Department of Biotechnology, K L University, Green fields, Vaddeswaram, Guntur Dist, Andhra Pradesh, India - 522502;

Email

jksingh@kluniversity.in;

Article Type

Hypothesis

Date

Received October 12, 2017; Accepted October 22, 2017; Published November 30, 2017

Ymr210w was identified as a MAG (Monoacylglycerol) lipase. The accumulation of the phospholipids in the ymr210wΔ was not clearly understood. It was expressed in S. cerevisiae using pYES2/CT vector and His-tag purified recombinant protein confirmed TAG lipase activity. To further evaluate the role of YMR210w, ester hydrolase activity was also confirmed with pNP-acetate, pNP-butyrate and pNP - palmitate. GC-MS lipid profiling of ymr210wΔ showed an increase in the 15:0 Pentadecanoic acid by 76% among the total lipids. Phospholipid, Erucic acid 22:1 (Δ13) showed 43% increase while steryl esters showed significant changes with 16:0 hexadecanoic acid augmentations by 80% and 18:0 Octadecanoic acid by 165% when compared to wild type (WT). Increase in the steryl ester and TAG content supports the accumulation of lipid bodies in ymr210wΔ strain when compared with WT cells.

Keywords

TAG Lipase, Ester Hydrolase, Lipid droplets, Saccharomyces cerevisiae, Triacylglycerols, Steryl esters.

Citation

Kumar et al. Bioinformation 13(11): 360-365 (2017)

Edited by

P Kangueane

ISSN

0973-2063

Publisher

Biomedical Informatics

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.