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Title

Epitope design of L1 protein for vaccine production against Human Papilloma Virus types 16 and 18

 

Authors

Sunanda Baidya1,*, Rasel Das2, Md. Golam Kabir1, Md. Arifuzzaman3.

 

Affiliation

1Department of Biochemistry & Molecular Biology, University of Chittagong, Chittagong 4331, Bangladesh; 2Leibniz Institute for Surface Modification, Permoserstraße 15, 04318 Leipzig, Germany; 3Department of Biochemistry and Biotechnology, University of Science and Technology Chittagong (USTC), Foy’s Lake, Chittagong 4202, Bangladesh;

 

Email

sunanda.cu.ms.biochemistry.08@gmail.com

 

Article Type

Hpothesis

 

Date

Received February 18, 2017; Revised March 22, 2017; Accepted March 23, 2017; Published March 31, 2017

 

Abstract

Cervical cancer accounts for about two-thirds of all cancer cases linked etiologically to Human Papilloma Virus (HPV). 15 oncogenic HPV types can cause cervical cancer, of which HPV16 and HPV18 combinedly account for about 70% of it. So, effective epitope design for the clinically relevant HPV types 16 and 18 would be of major medical benefit. Here, a comprehensive analysis is carried out to
predict the epitopes against HPV types 16 and 18 through “reverse vaccinology” approach. We attempted to identify the evolutionarily conserved regions of major capsid protein (L1) as well as minor capsid protein (L2) of HPV and designed epitopes within these regions. In this study, we analyzed about 49 and 27 sequences of HPV L2 and L1 proteins respectively. Since we found that the
intertype variability of L2 is higher than for L1 proteins, our analysis was emphasized on epitopes of L1 of HPV types 16 and 18. We had selected HLA-A*0201, DRB1*1501, DQB1*0602, DRB1*0401 and DQB1*0301 alleles for the prediction of T cell epitopes of L1 of HPV 16 and 18. Finally, we reported that predicted epitope sequences EEYDLQFIFQLCKITLTA, and RHGEEYDLQFIFQLCKITLTA of
L1 protein of HPV 16, and LPDPNKF, PETQRLVWAC, PVPGQYDA, YNPETQRLVWAC, DTGYGAMD, PVPGQYDATK, KQDIPKVSAYQYRVFRV, RDNVSVDYKQTQLCI and YSRHVEEYDLQFIF of L1 protein of HPV 18 could be therapeutic tools for vaccine design against HPV.

 

Keywords

Human Papilloma Virus, capsid proteins, cervical cancer.

 

Citation

Baidya et al. Bioinformation 13(3): 86-93 (2017)

 

Edited by

P Kangueane

 

ISSN

0973-2063

 

Publisher

Biomedical Informatics

 

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.