BACK TO CONTENTS   |    PDF   |    NEXT

Title

Viral diagnosis in Indian livestock using customized microarray chips

 

Authors

Brijesh S Yadav, Mayank Pokhriyal, Barkha Ratta, Ajay Kumar, Meeta Saxena & Bhaskar Sharma*

Affiliation

Division of Biochemistry, Indian Veterinary Research Institute, Izatnagar, India - 243122

Email

bhaskar@ivri.res.in; *Corresponding author

 

Article Type

Hypothesis

 

Date

Received September 14, 2015; Accepted October 09, 2015; Published November 30, 2015

 

Abstract

Viral diagnosis in Indian livestock using customized microarray chips is gaining momentum in recent years. Hence, it is possible to design customized microarray chip for viruses infecting livestock in India. Customized microarray chips identified BHV-1 (Bovine herpes virus-1), CAV-1 (Canine Adeno Virus-1), and CPV-2 (Canine Parvo Virus-2) in clinical samples. Microarray identified specific probes were further confirmed using RT-PCR in all clinical and known samples. Therefore, the application of microarray chips during viral disease outbreaks in Indian livestock is possible where conventional methods are unsuitable. It should be noted that customized application requires a detailed cost efficiency calculation.

 

Keywords

DNA microarray, viral diagnosis, livestock, RT-PCR (reverse transcriptase polymerase chain reaction).

 

Citation

Yadav et al. Bioinformation 11(11): 489-492 (2015)
 

Edited by

P Kangueane

 

ISSN

0973-2063

 

Publisher

Biomedical Informatics

 

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.