Title |
Viral diagnosis in Indian livestock using customized microarray chips
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Authors |
Brijesh S Yadav, Mayank Pokhriyal, Barkha Ratta, Ajay Kumar, Meeta Saxena & Bhaskar Sharma* |
Affiliation |
Division of Biochemistry, Indian Veterinary Research Institute, Izatnagar, India - 243122 |
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bhaskar@ivri.res.in; *Corresponding author
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Article Type |
Hypothesis
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Date |
Received September 14, 2015; Accepted October 09, 2015; Published November 30, 2015
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Abstract |
Viral diagnosis in Indian livestock using customized microarray chips is gaining momentum in recent years. Hence, it is possible to design customized microarray chip for viruses infecting livestock in India. Customized microarray chips identified BHV-1 (Bovine herpes virus-1), CAV-1 (Canine Adeno Virus-1), and CPV-2 (Canine Parvo Virus-2) in clinical samples. Microarray identified specific probes were further confirmed using RT-PCR in all clinical and known samples. Therefore, the application of microarray chips during viral disease outbreaks in Indian livestock is possible where conventional methods are unsuitable. It should be noted that customized application requires a detailed cost efficiency calculation.
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Keywords |
DNA microarray, viral diagnosis, livestock, RT-PCR (reverse transcriptase polymerase chain reaction).
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Citation |
Yadav
et al.
Bioinformation 11(11): 489-492 (2015) |
Edited by |
P Kangueane
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ISSN |
0973-2063
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Publisher |
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License |
This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.
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