Title

Authors

Affiliation

¹Department of Genetics and Bioengineering, International Burch University, Francuske revolucije BB, Ilidža 71000; ²Department of Clinical Pathology, Clinical Center of the University of Sarajevo, Bosnia and Herzegovina; ³Department of Information Technologies, International Burch University, Francuske revolucije BB, Ilidža 71000, Sarajevo, Bosnia and Herzegovina

Email

sdogan@ibu.edu.ba; *Corresponding author

Article Type

Hypothesis

Date

Received May 07, 2015; Revised May 13, 2015; Accepted June 16, 2015; Published June 30, 2015

The guanine rich locations are present in human genome. Previous studies have shown that the presence of G rich sequences and motifs may be significant for gene activity and function. We decided to focus our interest to identify G rich motifs in promoters of oncogenes and tumor suppressor genes. We used a set of 100 most common oncogenes and tumor suppressor genes (TSG) for this analysis. We collected 600nt long promoters with -500 and +100 TSS (transcription start site) from the oncogenes and TSG set. Using a computer program, we calculated the G densities using numbers and locations of G forms with 100nt moving widow. We included G numbers from 2 to 7 guanines. Analysis shows that G density increases from -500 to +100 and more from TSS. G density is found to be maximum within -/+100 of TSS. The results of G densities were compared with the expression data of the selected oncogenes and tumor suppressor genes in patients with colon cancer (n=174).

Citation

Dogan et al. Bioinformation 11(6): 290-295 (2015)
 

Edited by

P Kangueane

ISSN

0973-2063

Publisher

Biomedical Informatics

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.