Phyto-extraction of heavy metals and biochemical changes with Brassica nigra L. grown in rayon grade paper mill effluent irrigated soil



Uday Veer Singh1, Amar Abhishek2, Monika Bhaskar3, Neeraj Tandan4*, Nasreen Ghazi Ansari5 & Netra Pal Singh6



1Environmental Chemistry Division, CSIR-Indian Institute of Toxicology Research Centre, Post Box 80, M.G. Marg, Lucknow-226001, U. P., INDIA; 2Department of Urology, King George’s Medical University, Lucknow 226003, U.P, INDIA; 3Department of Botany, Guru Ghasidas Vishwavidyalaya, Bilaspur-495009, Chhattisgarh, INDIA; 4Scientific and Applied Research Center Post Box 2241, Meerut- 250001, U. P., INDIA; 5Metal Analysis Laboratory, CSIR- Indian Institute of Toxicology Research, Post Box No. 80, Mahatma Gandhi Marg, Lucknow Pin- 226001, INDIA; 6Bioinorganic Chemistry Division, Department of Chemistry, Meerut College, Meerut, India


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Received January 09, 2015; Accepted February 05, 2015; Published March 31, 2015



In this study, distribution of metal accumulation and their biological changes of Indian mustard plants (Brassica nigra L.) grown in soil irrigated with different concentration of rayon grade paper effluent (RGPE, 25%, 50%, 75%, 100%, v/v) were studied. A pronounced effect was recorded at 50% (v/v) RGPE on germination of seeds, amylase activity and other growth parameters in Indian mustard plants. An increase in the chlorophyll and protein contents was also recorded at <50% (v/v) RGPE followed by a decrease at higher concentrations of RGPE (>75%). A significant increase lipid peroxidation was recorded, which was evidenced by the increased malondialdehyde (MDA) content in shoot, leaves and seeds in tested plant at all the concentrations of RGPE. This Indian mustard plants (Brassica nigra L.) are well adapted for tolerance of significant amount of heavy metals due to increased level of antioxidants (cysteine and ascorbic acid) in root shoot and leaves of treated plants at all concentration of RGPE. Moreover, it is also important that RGPE should be treated to bring down the metal concentration well within the prescribed limit prior to use in agricultural soil for ferti-irrigation.



Rayon grade paper mill effluent, Indian mustard plant, Phytoremediation, Heavy metals, Lipid peroxidation, biochemical parameters.



Singh et al.   Bioinformation 11(3): 138-144 (2015)

Edited by

P Kangueane






Biomedical Informatics



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