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Title

In vitro Osteogenic impulse effect of Dexamethasone on periodontal ligament stem cells

 

Authors

Mohamad Ali Roozegar1, Tayebeh Malek Mohammadi2, Mohamad Reza Havasian1, Jafar Panahi1, Amirreza Hashemian1, Mansur Amraei3 & Behzad Hoshmand4*

 

Affiliation

1Department of Periodentistry, Faculty of Dentistry, Ilam University of Medical Sciences, Ilam/ Iran; 2Research Center for Social Determinant of Health, Institute of Futures Studies in Health and dental public health department, Kerman University of Medical sciences, Kerman/ Iran; 3Department of Physiology, Faculty of Medicine, Ilam University of Medical Sciences, Ilam/ Iran; 4Department of Periodontics, Faculty of Dentistry, Shahid beheshti University of Medical Sciences, Tehran/ Iran

 

Email

Roozegar2015@gmail.com; *Corresponding author

 

Article Type

Hypothesis

 

Date

Received December 16, 2014; Accepted January 26, 2015; Published February 28, 2015

 

Abstract

Periodontium is a complex organ composed of mineralized epithelial and connective tissue. Dexamethasone could stimulate proliferation of osteoblast and fibroblasts. This study aimed to assess the osteogenic effect of dexamethasone on periodental ligament (PDL) stem cells. PDL stem cells were collected from periodontal ligament tissue of root of extracted premolar of young and healthy people. The stem cells were cultured in α-MEM Medium in three groups, one group with basic medium contains (α- MEM and FBS 10 % and 50 mmol of β_ gelisrophosphat and L_ ascorbic acid g/ml), the second group: basic medium with dexamethasone and the third one: basic medium without any osteogenic stimulant. Mineralization of cellular layer was analyzed with Alizarin red stain method. Osteogenic analysis was done by Alkaline phosphates and calcium test. These analysis indicated that the amount of intra-cellular calcium and alkaline phosphates in the Dexamethasone group was far more than the control and basic group (P<0.05). The results of Alizarin red stain indicated more mineralization of cultured cells in Dexamethasone group (P<0.05). The study results showed that Dexamethasone has significant osteogenic effect on PDL stem cells and further studies are recommended to evaluate its effect on treatment of bone disorders.

 

Keywords

PDL stem cell, dexamethasone, alizarin red, in vitro.

 

Citation

Roozegar et al. Bioinformation 11(2): 096-100 (2015)

 

Edited by

P Kangueane

 

ISSN

0973-2063

 

Publisher

Biomedical Informatics

 

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.