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Title |
16SrRNA sequencing of dye decolorizing bacteria isolated from soil
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Authors |
Avnish Kumar, Monika Asthana*, Poonam Gupta, Shweta Yadav, Deepti Sharma, Km. Neeraj Singh & Sunil Kumar |
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Affiliation |
Department of Biotechnology, School of Life Sciences, Khandari Campus Dr. Bhim Rao Ambedkar University, Agra-282004, U.P., India
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mailtomonikasaxena@gmail.com; *Corresponding author
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Article Type |
Hypothesis
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Date |
Received December 14, 2014; Accepted December 25, 2014; Published January 30, 2015
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Abstract |
Dye's residues in textile effluents are hazardous for humans and animals health. Such pollutants can be degraded into non-harmful molecules using biological approaches that are considered cheaper and ecologically safer. Isolated 15 bacterial cultures from soil that could be used in biological system were showed decolorization capacity for Acid Green dye (33.9% to 94.0%) using thin layer chromatography and broth culture method. The most promising cultures (AMC3) to decolorize Acid green Dye (94.6%) was re-coded as NSDSUAM for submitting at IMTECH, Chandigarh for sequencing. The 16SrRNA sequencing suggested that it can be a variant of Pseudomonas geniculata (99.85% identical similarity) with difference of 2 base pairs to reference strain Pseudomonas geniculata ATCC 19374(T). Thus present study proposed dye decolorizing efficiency of the isolated strain of Pseudomonas geniculata that was previously unnoticed. The sequence is deposited in NCBI GenBank with the accession number KP238100.
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Keywords |
Bacteria, pollutants, TLC, biodegradation, 16S rRNA sequencing, Azoreductase.
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Citation |
Kumar et al.
Bioinformation 11(1): 001-005 (2015) |
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Edited by |
P Kangueane
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ISSN |
0973-2063
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Publisher |
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License |
This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License. |