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Title

Genomic cloning and sequence analysis of Interleukin-10 from Labeo rohita

 

Authors

Sweta Karan1, Himani Kaushik1, Nipun Saini1, Pramod K Sahoo2, Aparna Dixit3 & Lalit C Garg1*

 

Affiliation

1Gene Regulation Laboratory, National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi 110 067, India; 2Fish Health Management Division, Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar 751 002, India; 3School of Biotechnology, Jawaharlal Nehru University, New Delhi 110 067, India

 

Email

lalit@nii.ac.in; lalitcgarg@gmail.com; *Corresponding author

 

Article Type

Hypothesis

 

Date

Received September 26, 2014; Accepted October 01, 2014; Published October 30, 2014

 

Abstract

Interleukin-10 (IL-10) is a pleiotropic cytokine and plays an important role in inflammation, immunoregulation and the pathogenesis of various diseases. Therefore, it is our interest to isolate, clone, sequence and characterize IL-10 gene from the fish Labeo rohita (Lr). The gene was amplified using genomic DNA isolated from head kidney with primers designed on conserved sequence homologues of fishes belonging to Cyprinidae family. The gLrIL-10 is 1467 nucleotides long with five exons and four introns sharing the same organization as of mammalian IL-10 genes. An open reading frame of 537 bp was found to encode a putative 179 amino acid protein with a signal peptide of 22 amino acids with conserved signature sequence motif. Sequence analysis showed similarity with the IL-10 from most fresh water fishes of Cyprinidae family. LrIL-10 has 27.2 % identity and 54.95 % similarity with the human IL-10. Sequence analysis followed by phylogenetic studies showed highest identity with Catla catla (98%) followed by Cyprinus carpio (93%), Hypophthalmichthys molitrix (89%) and is distantly related to human, rhesus monkey and frog. These data from primary sequence characterization may be used to further understand transcriptional regulation and functional characterization of LrIL-10 in relation to species-specific molecular immunology.

 

Keywords

Interleukin-10, Labeo rohita, cloning.

 

Abbreviations

IL-10, Interleukin-10, Lr, Labeo rohita; nt, nucleotides.

 

Citation

Karan et al. Bioinformation 10(10): 623-629 (2014)
 

Edited by

P Kangueane

 

ISSN

0973-2063

 

Publisher

Biomedical Informatics

 

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.