Title

Authors

Affiliation

¹Department of Biotechnology, Integral University, Dasauli, Kursi Road, Lucknow 226 026, Uttar Pradesh, India; ²Indian Institute of Sugarcane Research, Raibareli Road, P.O. Dilkusha, Lucknow - 226 002, Uttar Pradesh, India

Email

mohdashfaquekhan@rediffmail.com; *Corresponding author

Article Type

Hypothesis

Date

Received August 00, 2014; Accepted August 00, 2014; Published October 30, 2014

Sugarcane bagasse (SCB), a lignocellulosic byproduct of juice extraction from sugarcane, is rich in cellulose (40-42%). This could be used as a substrate for the production of cellulase complex. Fermentation conditions were optimized for production of cellulase complex (CMCase, Cellulobiase and FPase) by wild type Trichoderma sp. using sugarcane bagasse as sole carbon source. Alkaline treatment (2% NaOH) of bagasse (AlSCB) was found suitable for the production of reducing sugar over the acidic pretreatment method. After 5 days of incubation period, 5% substrate concentration at pH 5.0 and 400C resulted in maximum production of CMCase (0.622 U), while maximum (3.388 U) production of cellulobiase was obtained at 300C. The CMCase was precipitated and purified to the extent of 59.06 fold by affinity chromatography with 49.09% recovery. On 12% SDS-PAGE, a single band corresponding to 33 kDa was observed. The Km and Vmax for CMCase from Trichoderma was found 507.04 mg/ml and 65.32 mM/min, respectively. The enzyme exhibited maximum activity at 300C at pH-5.0 (0.363 U) and was stable over range of 20-600C and pH 5.0-7.5.

Citation

Ashfaque et al.   Bioinformation 10(10): 606-610 (2014)
 

Edited by

P Kangueane

ISSN

0973-2063

Publisher

Biomedical Informatics

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.