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Title

Analysis of proteins involved in the production of MAA’s in two Cyanobacteria Synechocystis PCC 6803 and Anabaena cylindrica

 

Authors

Md Akhlaqur Rahman, Sukrat Sinha, Shephali Sachan, Gaurav Kumar, Shailendra Kumar Singh & Shanthy Sundaram*

 

Affiliation

Centre of Biotechnology, University of Allahabad, Allahabad-211002, India

 

Email

shanthy_s@rediffmail.com; *Corresponding author

 

Article Type

Hypothesis

Date

Received May 19, 2014; Accepted May 26, 2014; Published June 22, 2014

 

Abstract

Mycosporine- like amino acids (MAAs) are small (<400Da), colourless, water soluble compounds composed of cyclohexenone or cyclohexinimine chromophere conjugated with the nitrogen substituent of amino acid or its amino alcohol. These compounds are known for their UV- absorbing role in various organisms and seem to have evolutionary significance. The biosynthesis of MAAs is presumed to occur via the first part of shikimate pathway. In the present work two cyanobacteria Synechocystis PCC 6803 and Anabaena cylindrica were tested for their ability to synthesize MAAs and protein involved in the production of MAAs. It was found that protein sequence 3-phosphoshikimate 1-carboxyvinyltransferase is involved in producing mycosporine glycine in Synechocystis PCC 6803 and 3-dehydroquinate synthase is involved for producing shinorine in Anabaena cylindrica. Phylogenetic and bioinformatic analysis of Mycosporine like amino acid producing protein sequence of both cyanobacterial species Synechocystis PCC 6803 and Anabaena cylindrica provide a useful framework to understand the relationship of the different forms and how they have evolved from a common ancestor. These products seem to be conserved but the residues are prone to variation which might be due the fact that different cyanobacteria show different physiological process in response of Ultraviolet stress.

 

Keywords

Cyanobacteria, Mycosporine- like amino acids (MAAs), Mycosporine glycine, Shinorine, Ultraviolet

 

Citation

Rahman  et al. Bioinformation 10(7): 449-453 (2014)
 

Edited by

P Kangueane

 

ISSN

0973-2063

 

Publisher

Biomedical Informatics

 

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.