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Title

Multiple antibiotic resistances in metal tolerant E. coli from hospital waste water

 

Authors

Manzar Alam & Mohd Imran*

 

Affiliation

Department of Biosciences, Integral University, Lucknow, India-226026

 

Email

imranmohdkhan@rediffmail.com; *Corresponding authors

 

Article Type

Hypothesis

 

Date

Received April 19, 2014; Accepted April 20, 2014; Published May 20, 2014

 

Abstract

Study of antibiotic resistance was done among the metal tolerant E. coli isolates from hospital wastewater at Lucknow city. Metal tolerance was determined in terms of visible growth on metal amended plates at their varying concentrations. MICs were also determined among all metal tolerant E. coli isolates. All the isolates showed their MIC in between 100-2000 g/ml while maximum isolates demonstrated their MICs at 400, 800 and 1600 g/ml against all the metal tested. 23.07% of the isolates showed their MIC at 2000 g/ml against Ni3+. Multiple antibiotic resistances were recorded among all the metal resistant E.coli isolates. A high level of resistance was observed against Methicillin (86.53%) followed by penicillin (73.07%), Cephradin (57.69%), Rifampicin (34.61%), Erythromycin (26.92%), Nalidixic acids (25%), Chloramphenicol (3.84%) and least to Gentamycine (1.92%). Streptomycin was recorded most effective against E.coli isolates among the entire antibiotic tested. Antimicrobial resistance observed among the bacteria from the aquatic system contaminated with hospital wastes may be threatful for the environment and public health both.

 

Keywords

Heavy metals, Antibiotics, Escherichia coli, Hospital wastewater

 

Citation

Alam & Imran, Bioinformation 10(5): 267-272 (2014)
 

Edited by

P Kangueane

 

ISSN

0973-2063

 

Publisher

Biomedical Informatics

 

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.