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Title

Study on Folate Binding Domain of Dihydrofolate Reductase in Different Plant species and Human beings

 

Authors

Aveek Samanta1, Animesh Kumar Datta1 & Siraj Datta2*

 

Affiliation

1Department of Botany, Cytogenetics, Genetics and Plant Breeding Section, Kalyani University, Kalyani-741235, West Bengal, India; 2Department of Biotechnology, Haldia Institute of Technology, Haldia-721657, West Bengal, India

 

Email

dattasiraj@gmail.com; *Corresponding author

 

Article Type

Database

Date

Received January 10, 2014; Accepted January 25, 2014; Published February 19, 2014

 

Abstract

Data base (NCBI and TIGR) searches are made to retrieve protein sequences of different plant species namely Medicago truncatula, Pisum sativum, Ricinus communis, Arabidopsis thaliana, Vitis vinifera, Glycine max, Daucus carota, Oryza sativa Japonica Group, Arabidopsis lyrata subsp. lyrata, Brachypodium distachyon, Oryza sativa Indica Group, Zea mays and careful alignment of derived sequences shows 95% or higher identity. Similarly, DHFR sequence of human being is also retrieved from NCBI. A phylogenetic tree is constructed from different plant and human DHFR domain using the Neighbour Joining method in MEGA 5.05. Conservation score is performed by using PARALINE. Result suggests that folate binding domain of dihydrofolare reductase is conserved (score 8.06) and excepting some minor variations the basic structure of the domain in both plant species and human being is rather similar. Human DHFR domain contains PEKN sequence near active site, though proline is common for all the selected organisms but the other sequences are different in plants. The plant domain is always associated with TS (Thymidylate synthase). Plant based system is predicted to be an effective model for assessment of MTX (Methotrexate) and other antifolate drugs.

 

Keywords

DHFR, Methotrexate, phylogenetic tree.

 

Citation

Samanta  et al. Bioinformation 10(2): 101-104 (2014)
 

Edited by

P Kangueane

 

ISSN

0973-2063

 

Publisher

Biomedical Informatics

 

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.