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Title |
Isolation and In-silico characterization of Peroxidase isoenzymes from Wheat (Triticum aestivum) against Karnal Bunt (Tilletia indica) |
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Authors |
Shalini Purwar*, Ankit Gupta, Geetika Vajpayee & Shanthy Sundaram |
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Affiliation |
Center for Biotechnology University of Allahabad, Allahabad
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purwarshalini@gmail.com; *Corresponding author
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Article Type |
Hypothesis
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Date |
Received January 16, 2014; Accepted January 26, 2014; Published February 19, 2014
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Abstract |
To investigate the role of Peroxidase and its physiological significance under Karnal Bunt (KB) were determined in resistant (HD-29) and susceptible genotype (WH-542) of wheat during different developmental stages. The enzymes were expressed constitutively in both the susceptible and resistant genotype. In gel assay and differential expression analysis of POD was significantly higher (p >0.05) in Sv and S2, than the S1 and S3 stages. In silico analysis of Peroxidase for eg. physico-chemical properties, secondary structural features and phylogenetic classification for comparative analysis. Motif and Domain analysis of Peroxidase by MEME, to be important for the biological functions, and studies of evolution. Our results clearly indicate that the enhanced expression of POD at the WS2 stage, which reinforces its role in stage dependent immunity against Karnal bunt and role of POD metabolism provides genotype and stage dependant structural barrier resistance in wheat against KB.
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Keywords |
Peroxidase (POD), Karnal Bunt, Triticum, insilico analysis, Fungus
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Citation |
Purwar et al.
Bioinformation 10(2): 087-093 (2014) |
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Edited by |
P Kangueane
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ISSN |
0973-2063
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Publisher |
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License |
This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License. |