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Title

In silico identification of cross affinity towards Cry1Ac pesticidal protein with receptor enzyme in Bos taurus and sequence, structure analysis of crystal proteins for stability

 

Authors

King Solomon Ebenezer1, Ramesh Nachimuthu2, Prabha Thiagarajan3 & Rajesh Kannan Velu1*

 

Affiliation

1Rhizosphere Biology Laboratory, Department of Microbiology, Bharathidasan University, Tiruchirappalli - 620024, TamilNadu, India; 2School of Biosciences and Technology, Vellore Institute of Technology, Vellore-632014, TamilNadu, India; 3Department of Microbiology, JJ College of Arts and Science, Pudukkottai - 622422, TamilNadu, India

 

Email

uvrajesh@gmail.com; *Corresponding author

 

Article Type

Hypothesis

 

Date

Received July 09, 2013; Revised July 15, 2013; Accepted July 16, 2013; Published August 28, 2013

 

Abstract

Any novel protein introduced into the GM crops need to be evaluated for cross affinity on living organisms. Many researchers are currently focusing on the impact of Bacillus thuringiensis cotton on soil and microbial diversity by field experiments. In spite of this, in silico approach might be helpful to elucidate the impact of cry genes. The crystal a protein which was produced by Bt at the time of sporulation has been used as a biological pesticide to target the insectivorous pests like Cry1Ac for Helicoverpa armigera and Cry2Ab for Spodoptera sp. and Heliothis sp. Here, we present the comprehensive in silico analysis of Cry1Ac and Cry2Ab proteins with available in silico tools, databases and docking servers. Molecular docking of Cry1Ac with procarboxypeptidase from Helicoverpa armigera and Cry1Ac with Leucine aminopeptidase from Bos taurus has showed the 125th amino acid position to be the preference site of Cry1Ac protein. The structures were compared with each other and it showed 5% of similarity. The cross affinity of this toxin that have confirmed the earlier reports of ill effects of Bt cotton consumed by cattle.

 

Keywords

Bacillus thuringiensis, Crystal protein, Docking, Phylogenetic analysis, Persistence, Cross affinity.

 

Citation

Ebenezer et al.   Bioinformation 9(15): 792-795 (2013)

 

Edited by

P Kangueane

 

ISSN

0973-2063

 

Publisher

Biomedical Informatics

 

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.