Analysis of gene expression on ngn3 gene signaling pathway in endocrine pancreatic cancer

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Title	Analysis of gene expression on ngn3 gene signaling pathway in endocrine pancreatic cancer
Authors	Prashantha Nagaraja¹*, Kavya Parashivamurthy², Nandini Sidnal², Siddappa Mali², Dakshyani Nagaraja¹, Sivarami Reddy³
Affiliation	¹Department of Bioinformatics, Scientific Bio-Minds, Bangalore-560092; ²Department of Biotechnology, KLE Dr.M.S. Sheshagiri College of Engineering and Technology, Belgaum-590008; 3Department of Biotechnology, Scientific Bio-Minds, Bangalore-560092
Email	prashanthacn@gmail.com; *Corresponding author
Article Type	Hypothesis
Date	Received July 17, 2013; Revised July 20, 2013; Accepted July 20, 2013; Published August 07, 2013
Abstract	In order to define the undifferentiated transcriptional factors present in neurogenesis of pancreatic β-islet cells, we studied the effect of Pdx1 in embryonic stem cell derived endocrine lineage. There are undifferentiated transcriptional progenitors Pdx1+/Ptf1a+/Cpa1+ tracking the growth of acini, ducts, α and β-islet cells. The upregulated transcriptional factors Pdx1 and ngn3 specify consequences of cell cycle regulation in early gut endocrine cells. The undifferentiated transcriptional factors basic helix loop helix (bHLH) protein regulate Ptf1a+/Cpa1+ in acini, ducts and it also regulate ngn3 to decrease expression of insulin and other pancreas secretions. The Pdx1+ and other unknown gene mutations show abnormal growth of neurogenesis in endocrine lineages. Using microarray based gene expression analysis to determine undifferential gene ontology in tissue specific gene regulation and disease progression that common in both metabolic and biological signaling pathways. The data expression profiles of ngn3 of wild- type pancreatic islet and islet derived tumor stem cells provide information on endocrine specific ngn3 genes. Therefore, 3755 genes were significantly regulated by Ngn3 induced pancreatic islet cell development. Moreover 317 upregulated and 175 downregulated, 757 genes deemed as undifferential expressions in endocrine cell. Furthermore to predict signaling pathways that associates with diabetes is highlighted.
Keywords	Pancreatic regeneration, neurogenesis, ngn3, bHLH, Notch Pathway, Embryonic stem cells, islet cells, pancreatic beta cells, diabetes, microarray.
Citation	Nagaraja et al. Bioinformation 9(14): 739-747 (2013)
Edited by	P Kangueane
ISSN	0973-2063
Publisher	Biomedical Informatics
License	This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.