Isolation and characterization of ethanol tolerant yeast strains

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Title	Isolation and characterization of ethanol tolerant yeast strains
Authors	Chiranjeevi Tikka¹, Hari Prasad Osuru¹, Navya Atluri², Praveen Chakravarthi Veera Raghavulu¹, Nanda Kumar yellapu¹, Ismail Shaik Mannur¹, Uppu Venkateswara Prasad³, Sudheer Aluru¹, Narasimha Varma K¹ & Matcha Bhaskar¹*
Affiliation	¹Division of Animal Biotechnology, Dept. of Zoology, S.V. University, Tirupati, AP, India; ²Department of Applied Microbiology, Sri Padmavati Mahila Visvavidyalayam, Tirupati, AP, India; ³Department of Biotechnology, Sri Venkateswara Institute of Medical Sciences, Tirupati, A.P, India
Email	matchabhaskar2010@gmail.com; *Corresponding authors
Article Type	Hypothesis
Date	Received March 31, 2013; Accepted April 01, 2013; Published April 30, 2013
Abstract	Yeast strains are commonly associated with sugar rich environments. Various fruit samples were selected as source for isolating yeast cells. The isolated cultures were identified at Genus level by colony morphology, biochemical characteristics and cell morphological characters. An attempt has been made to check the viability of yeast cells under different concentrations of ethanol. Ethanol tolerance of each strain was studied by allowing the yeast to grow in liquid YEPD (Yeast Extract Peptone Dextrose) medium having different concentrations of ethanol. A total of fifteen yeast strains isolated from different samples were used for the study. Seven strains of Saccharomyces cerevisiae obtained from different fruit sources were screened for ethanol tolerance. The results obtained in this study show a range of tolerance levels between 7%-12% in all the stains. Further, the cluster analysis based on 22 RAPD (Random Amplified polymorphic DNA) bands revealed polymorphisms in these seven Saccharomyces strains.
Keywords	Yeast, Ethanol, RAPD, Polymorphism
Citation	*Tikka et al.* Bioinformation 9(8): 421-425 (2013)
Edited by	P Kangueane
ISSN	0973-2063
Publisher	Biomedical Informatics
License	This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.