Isolation and Identification of hydrocarbon degrading bacteria from Ennore creek

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Title	Isolation and Identification of hydrocarbon degrading bacteria from Ennore creek
Authors	Mamitha Kumar Subathra¹*, Grasian Immanuel² & Albert Haridoss Suresh¹
Affiliation	¹Department of Microbiology, SRM Arts & Science College, Kattangulathur - 603203, Kancheepuram Dist, Tamilnadu, India; ²Centre for Marine Sciences & Technology, Manonmaniam Sundaranar University, Rajakaamangalam, KanyaKumari District, India.
Email	mamitha28@yahoo.com; *Corresponding author
Article Type	Hypothesis
Date	Received January 09, 2013; Accepted January 15, 2013; Published February 06, 2013
Abstract	The widespread problem caused due to petroleum products, is their discharge and accidental spillage in marine environment proving to be hazardous to the surroundings as well as life forms. Thus remediation of these hydrocarbons by natural decontamination process is of utmost importance. Bioremediation is a non-invasive and cost effective technique for the clean-up of these petroleum hydrocarbons. In this study we have investigated the ability of microorganisms present in the sediment sample to degrade these hydrocarbons, crude oil in particular, so that contaminated soils and water can be treated using microbes. Sediments samples were collected once in a month for a period of twelve months from area surrounding Ennore creek and screened for hydrocarbon degrading bacteria. Of the 113 crude oil degrading isolates 15 isolates were selected and cultivated in BH media with 1% crude oil as a sole carbon and energy source. 3 efficient crude oil bacterial isolates Bacillus subtilis I1, Pseudomonas aeruginosa I5 and Pseudomonas putida I8 were identified both biochemically and phylogenetically. The quantitative analysis of biodegradation is carried out gravimetrically and highest degradation rate, 55% was recorded by Pseudomonas aeruginosa I5 isolate.
Citation	*Subathra et al.* Bioinformation 9(3): 150-157 (2013)
Edited by	P Kangueane
ISSN	0973-2063
Publisher	Biomedical Informatics
License	This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.