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Title

A structural insight into the prokaryotic heat shock transcription regulatory protein s32: an implication of s32-DnaK interaction

 

Authors

Sourav Singha Roy, Monobesh Patra, Rakhi Dasgupta* & Angshuman Bagchi*

 

Affiliation

Department of Biochemistry and Biophysics, University of Kalyani, Kalyani, West Bengal, India.

 

Email

rdgadg@gmail.com; angshuman_bagchi@yahoo.com; *Corresponding authors

 

Article Type

Hypothesis

 

Date

Received October 06, 2012; Accepted October 16, 2012; Published October 31, 2012

 

Abstract

The heat shock response mechanism is a very vital biochemical process and is mainly controlled by s32 protein. The function of s32 is temperature dependent and at lower temperatures s32 is inactivated by its interactions with DnaK. This interaction is completely abolished above 42C till date no molecular details of the interactions are available. In the present scenario, an attempt has been made to analyze first the predicted structure of s32 obtained by comparative modeling techniques and then to study the interactions between s32 and DnaK. From this molecular modeling study we could specifically identify the binding sites of the interactions of s32 with DnaK which will enlighten the mechanism of regulation of its activity and stability by DnaK. Our study provides the idea for future mutational experiments in order to find out the possible roles of the amino acids of region2 and region3 of s32 in stability as well as in binding with DnaK.

 

Keywords

Heat Shock Protein, Protein-Protein Interaction, s32, DnaK, Docking, Molecular Dynamics Simulations

 

Citation

Roy et al. Bioinformation 8(21): 1026-1029 (2012)
 

Edited by

P Kangueane

 

ISSN

0973-2063

 

Publisher

Biomedical Informatics

 

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.