Title

Authors

Affiliation

¹Department of Pharmacology & Therapeutics, Chhatrapati Shahuji Maharaj Medical University, Chowk Lucknow-226003, Uttar Pradesh, India; ²Bioinformatics Centre, Biotech Park, Sector-G, Jankipuram, Lucknow-226021, Uttar Pradesh, India.

Email

saritasingh.bi@gmail.com; *Corresponding author

Article Type

Hypothesis

Date

Received June 23, 2012; Accepted July 26, 2012; Published August 24, 2012

Hepatitis D is a liable reason of mortality and morbidity worldwide. It is caused by an RNA virus known as Hepatitis Delta Virus (HDV). Genetic studies of HDV have shown that delta antigen protein is responsible for replication of genome and play a foremost role in viral infection. Therefore, delta antigen protein may be used as suitable target for disease diagnosis. Viral activity can be restrained through RNA interference (RNAi) technology, an influential method for post transcriptional gene silencing in a sequence specific manner. However, there is a genetic variability in different viral isolates; it is a great challenge to design potential siRNA molecules which can silence the respective target genes rather than any other viral gene simultaneously. In current study two effective siRNA molecules for silencing of HDV were rationally designed and validated using computational methods, which may lead to knockdown the activity of virus. Thus, t his approach may provide an insight for the chemical synthesis of antiviral RNA molecule for the treatment of hepatitis D, at genome level.

Keywords

Antiviral, RNAi, siRNA, Thermodynamics

Citation

Singh et al. Bioinformation 8(16): 749-757 (2012)
 

Edited by

P Kangueane

ISSN

0973-2063

Publisher

Biomedical Informatics

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.