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Title

Cloning and characterization of two neuropeptide genes from cereal cyst nematode, Heterodera avanae from India

 

Authors

Prasoon Thakur1, Amita Sharma1, Sashi B Rao2, Mukesh Kumar1, Nagavara Prasad G1, Nidhi Tyagi1, Divya Kamaraju1, Pradeep Papolu1, Prakash Banakar1 & Uma Rao1*

 

Affiliation

1Division of Nematology, Indian Agricultural Research Institute (IARI), New Delhi-110012; 2The Ella Foundation, Hyderabad, India-500039.

 

Email

umarao@iari.res.in; *Corresponding author

 

Article Type

Hypothesis

 

Date

Received June 19, 2012; Accepted June 26, 2012; Published July 06, 2012

 

Abstract

The cereal cyst nematode, Heterodera avenae (Wollenweber, 1924) is one of the most important plant parasitic nematodes of cereals. It is an obligate sedentary endo parasite causing considerable crop losses in wheat, barley and oats worldwide. FMRFamide-like peptides (FLPs) play critical role as neurotransmitters or neuromodulators in the nervous system and proposed as one of the important targets for the plant parasitic nematode management. Therefore, for the first time we have cloned and characterized two neuropeptide genes (flp-12 and flp-16) from the cDNA library of feeding female of H. avenae. Sequence analysis of FLPs revealed that both the neuropeptides are closely related with the parasitic as well as free-living nematodes. The flp-12 contains putative 22 residue long signal peptide at N-terminal suggesting its association with extra-cellular functions, while flp-16 does not contain signal peptide. Besides this, we have found highly conserved motif KFEFIRF in flp-12 and RFGK motif in flp-16. These two flp genes could be interesting and potential targets for functional validation to explore their utility for designing management strategies.

 

Citation

Thakur et al. Bioinformation 8(13): 617-621 (2012)
 

Edited by

P Kangueane

 

ISSN

0973-2063

 

Publisher

Biomedical Informatics

 

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.