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Title

Diversity of Streptomyces spp. in Eastern Himalayan region computational RNomics approach to phylogeny

 

Authors

Kaushik Bhattacharjee, Subhro Banerjee & Santa Ram Joshi*

 

Affiliation

Microbiology Laboratory, Department of Biotechnology and Bioinformatics, North-Eastern Hill University, Shillong-793022

 

Email

srjoshi2006@yahoo.co.in; *Corresponding author

 

Article Type

Hypothesis

 

Date

Received June 05, 2012; Accepted June 08, 2012; Published June 28, 2012

Abstract

Isolation and characterization of actinomycetes from soil samples from altitudinal gradient of North-East India were investigated for computational RNomics based phylogeny. A total of 52 diverse isolates of Streptomyces from the soil samples were isolated on four different media and from these 6 isolates were selected on the basis of cultural characteristics, microscopic and biochemical studies. Sequencing of 16S rDNA of the selected isolates identified them to belong to six different species of Streptomyces. The molecular morphometric and physico-kinetic analysis of 16S rRNA sequences were performed to predict the diversity of the genus. The computational RNomics study revealed the significance of the structural RNA based phylogenetic analysis in a relatively diverse group of Streptomyces.

 

Keywords

Actinobacteria, Eastern Himalaya, Streptomyces, 16S rDNA, Molecular morphometrics

 

Citation

Bhattacharjee et al. Bioinformation 8(12): 548-554 (2012)
 

Edited by

P Kangueane

 

ISSN

0973-2063

 

Publisher

Biomedical Informatics

 

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.