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Title |
Diversity of Streptomyces spp. in Eastern Himalayan region – computational RNomics approach to phylogeny |
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Authors |
Kaushik Bhattacharjee, Subhro Banerjee & Santa Ram Joshi* |
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Affiliation |
Microbiology Laboratory, Department of Biotechnology and Bioinformatics, North-Eastern Hill University, Shillong-793022
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srjoshi2006@yahoo.co.in; *Corresponding author
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Article Type |
Hypothesis
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Date |
Received June 05, 2012; Accepted June 08, 2012; Published June 28, 2012 |
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Abstract |
Isolation and characterization of actinomycetes from soil samples from altitudinal gradient of North-East India were investigated for computational RNomics based phylogeny. A total of 52 diverse isolates of Streptomyces from the soil samples were isolated on four different media and from these 6 isolates were selected on the basis of cultural characteristics, microscopic and biochemical studies. Sequencing of 16S rDNA of the selected isolates identified them to belong to six different species of Streptomyces. The molecular morphometric and physico-kinetic analysis of 16S rRNA sequences were performed to predict the diversity of the genus. The computational RNomics study revealed the significance of the structural RNA based phylogenetic analysis in a relatively diverse group of Streptomyces.
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Keywords |
Actinobacteria, Eastern Himalaya, Streptomyces, 16S rDNA, Molecular morphometrics
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Citation |
Bhattacharjee et al.
Bioinformation 8(12): 548-554 (2012) |
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Edited by |
P Kangueane
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ISSN |
0973-2063
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Publisher |
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License |
This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License. |