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Title |
Automation of a primer design and evaluation pipeline for subsequent sequencing of the coding regions of all human Refseq genes |
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Authors |
Daniel Lai1 & Donald R Love1, 2* |
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Affiliation |
1School of Biological Sciences, University of Auckland, Private Bag 92019, Auckland 1142, New Zealand; 2Diagnostic Genetics, LabPlus, Auckland City Hospital, PO Box 110031, Auckland 1148, New Zealand.
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donald@adhb.govt.nz; *Corresponding author
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Article Type |
Hypothesis
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Date |
Received April 23, 2012; Accepted April 26, 2012; Published April 30, 2012
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Abstract |
Screening for mutations in human disease-causing genes in a molecular diagnostic environment demands simplicity with a view to allowing high throughput approaches. In order to advance these requirements, we have developed and applied a primer design program, termed BatchPD, to achieve the PCR amplification of coding exons of all known human Refseq genes. Primer design, in silico PCR checks and formatted primer information for subsequent web-based interrogation are queried from existing online tools. BatchPD acts as an intermediate to automate queries and results processing and provides exon-specific information that is summarised in a spreadsheet format.
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Keywords |
Primers, Refseq genes, single nucleotide polymorphisms, BatchPD
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Citation |
Lai & Love,
Bioinformation 8(8): 365-368 (2012) |
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Edited by |
P Kangueane
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ISSN |
0973-2063
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Publisher |
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License |
This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License. |