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Title |
Isolation and characterization of protease producing bacteria from upper respiratory tract of wild chicken |
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Authors |
Sangeeta Raut1, Sudip Kumar Sen1*, Naveed Alam Kabir1, Soumya Satpathy1, Smita Raut2 |
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Affiliation |
1Department of Biotechnology, Gandhi Institute of Engineering and Technology, Gunupr,Rayagada,Odisha-765022,India; 2M.S Swaminathan Research Foundation, Jeypore, Koraput, Odisha, India.
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sudip.bio@gmail.com; *Corresponding author
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Article Type |
Hypothesis
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Date |
Received March 22, 2012; Accepted April 05, 2012; Published April 13, 2012 |
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Abstract |
Bacterial samples isolated from the upper respiratory tract of a healthy broiler chicken and a wild chicken suffering from influenza which were collected locally revealed proteolytic activity as detected by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and zymogram analysis. Among five protease producing strains screened, one was selected as promising protease producer. The activity of the protease produced by this organism is stable up to 620C. Optimum yield was achieved after 19 hours of culture, at pH 9.0 and 450C. The desired protein was precipitated from the crude extract by using ammonium sulfate (60%) followed by dialysis and purified by Ion-exchange chromatography. Further investigations are needed to know about the structure elucidation of the purified protein for industrial exploitation.
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Keywords |
Protease, SDS-PAGE, Zymogram, Gunupur. |
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Citation |
Raut et al.
Bioinformation 8(7): 326-330 (2012) |
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Edited by |
P Kangueane
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ISSN |
0973-2063
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Publisher |
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License |
This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License. |