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Title |
Optimization of DNA extraction from seeds and leaf tissues of Chrysanthemum (Chrysanthemum indicum) for polymerase chain reaction |
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Authors |
Saba Hasan*, Jyoti Prakash, Abhinav Vashishtha, Agnivesh Sharma, Kuldeep Srivastava, Faizuddin Sagar, Nausheen Khan, Keshav Dwivedi, Payal Jain, Saransh Shukla, Swati Prakash Gupta & Saumya Mishra |
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Affiliation |
Amity Institute of Biotechnology, Amity University, Viraj Khand-5, Gomtinagar, Lucknow (U.P.) - India 226010.
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saba_hasan2001@yahoo.com; *Corresponding author
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Article Type |
Hypothesis
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Date |
Received February 15, 2012; Accepted February 18, 2012; Published March 17, 2012
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Abstract |
Chrysanthemums constitute approximately 30 species of perennial flowering plants, belonging to the family Asteraceae, native to Asia and Northeastern Europe. Chrysanthemum is a natural cosmetic additive extracted from Chinese herb by modern biochemical technology. It has the properties of anti-bacterial, anti-viral, reducing (detoxification) and anti-inflammation. It possesses antioxidant characteristics, which could assist in minimizing free-radical induced damage. Therefore, it is widely used in skin and hair care products. Chemical composition of this herbal remedy includes kikkanols, sesquiterpenes, flavonoids, various essential oils containing camphor, cineole, sabinol, borneole and other elements that interfere with DNA, causing erroneous or no PCR products. In the present study, testing and modification of various standard protocols for isolation of high-quality DNA from leaf tissues and seeds of C. indicum was done. It was observed that the DNA obtained from seeds and leaf tissues with a modified cetyltrimethylammonium bromide buffer protocol was of good quality, with no colored pigments and contaminants. Also, DNA could be extracted from leaf tissues without using liquid nitrogen. Quality of DNA extracted from seeds was much better as compared to that extracted from leaf tissues. The extracted DNA was successfully amplified by PCR using arbitrary RAPD primers. The same protocol will probably be useful for extraction of high-molecular weight DNA from other plant materials containing large amounts of secondary metabolites and essential oils.
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Keywords |
Cetyl trimethyl ammonium bromide, PCR Amplification, secondary metabolites, EDTA, Chrysanthemum indicum
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Citation |
Hasan et al.
Bioinformation 8(5): 225-228 (2012) |
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Edited by |
P Kangueane
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ISSN |
0973-2063
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Publisher |
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License |
This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License. |