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Title

Antigenic variability in Neuraminidase protein of Influenza A/H3N2 vaccine strains (1968 2009)

 

Authors

Pratip Shil*, Sameer S Chavan, Sarah S Cherian

 

Affiliation

Bioinformatics and Data Management Division, National Institute of Virology, 20A Dr. Ambedkar Road, Pune- 411001, India

 

Email

shilpratip@gmail.com, pshil@icmr.org.in; *Corresponding author

 

Phone

+91 20 26006218

 

Fax

+91 20 26122669

 

Article Type

Hypothesis

 

Date

Received July 23, 2011; Accepted July 25, 2011; Published September 06, 2011

 

Abstract

Antigenic drift and shift involving the surface proteins of Influenza virus gave rise to new strains that caused epidemics affecting millions of people worldwide over the last hundred years. Variations in the membrane proteins like Hemagglutinin (HA) and Neuraminidase (NA) necessitates new vaccine strains to be updated frequently and poses challenge to effective vaccine design. Though the HA protein, the primary target of the human immune system, has been well studied, reports on the antigenic variability in the other membrane protein NA are sparse. In this paper we investigate the molecular basis of antigenic drift in the NA protein of the Influenza A/H3N2 vaccine strains between 1968 and 2009 and proceed to establish correlation between antigenic drift and antigen-antibody interactions. Sequence alignments and phylogenetic analyses were carried out and the antigenic variability was evaluated in terms of antigenic distance. To study the effects of antigenic drift on the protein structures, 3D structure of NA from various strains were predicted. Also, rigid body docking protocol has been used to study the interactions between these NA proteins and antibody Mem5, a 1998 antibody.

 

Keywords

Influenza virus, H3N2, neuraminidase, antibody, molecular docking, antigenic drift, host-pathogen interaction, Mem5.

 

Citation

Shil et al. Bioinformation 7(2): 76-81 (2011)
 

Edited by

P Kangueane

 

ISSN

0973-2063

 

Publisher

Biomedical Informatics

 

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.