Title

Authors

Affiliation

Centre of Biotechnology, School of Pharmaceutical Sciences, Siksha O Anusandhan University, Bhubaneswar-751003, India

Email

sanghamitran@yahoo.com; *Corresponding author

Phone

09437061976

Article Type

Hypothesis

Date

Received July 07, 2011; Accepted July 12, 2011; Published July 19, 2011

Resistance genes are among the most important gene classes for plant breeding purposes being responsible for activation of plant defense mechanisms. Among them, the nucleotide binding site-leucine rich repeat (NBS-LRR) class R-genes are the most abundant and actively found in all types of plants. Insilico characterization of EST database resulted in the detection of 28 NBS types R-gene sequences in Curcuma longa. All the 28 sequences represented the NB-ARC domain, 21 of which were found to have highly conserved motif characteristics and categorized as regular NBS genes. The Open Reading Frames varied from 361 (CL.CON.3566) to 112 (CL.CON.1267) with an average of 279 amino acids. Most alignment occurred with monocots (67.8%) with emphasis on Oryza sativa and Zingiber sequences. All best alignments with dicots occurred with Arabidopsis thaliana, Populus trichocarpa and Medicago sativa. These detected NBS type R-genes from Curcuma longa can be used as a valuable resource for molecular marker development, molecular mapping of R-genes, and identification of resistance gene analogs and functional and evolutionary characterization of NBS–LRR-encoding resistance genes in asexually reproducing plants.

Keywords

Curcuma longa, expressed sequence tags, NBS-LRR, R-genes, TBLASTN

Citation

Joshi et al. Bioinformation 6(9): 360-363 (2011)
 

Edited by

P Kangueane

ISSN

0973-2063

Publisher

Biomedical Informatics

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.