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Title

Structural prediction and comparative docking studies of psychrophilic - Galactosidase with lactose, ONPG and PNPG against its counter parts of mesophilic and thermophilic enzymes

 

Authors

Ponnada Suresh Kumar1, KK Pulicherla2, Mrinmoy Ghosh1, Anmol Kumar1, KRS Sambasiva Rao1*

 

Affiliation

1Department of Biotechnology, Acharya Nagarjuna University, Nagarjuna Nagar-522110, Guntur, Andhra Pradesh, India; 2Department of Biotechnology, R.V.R. & J.C. College of Engineering, Chowdavaram, Guntur-522019, Andhra Pradesh, India

 

Email

krssrao@gmail.com; krssrao@gmail.com *Corresponding author

 

Article Type

Hypothesis

 

Date

Received June 20, 2011; Accepted June 28, 2011; Published July 06, 2011

 

Abstract

Enzymes from psychrophiles catalyze the reactions at low temperatures with higher specific activity. Among all the psychrophilic enzymes produced, cold active -galactosidase from marine psychrophiles revalorizes a new arena in numerous areas at industrial level. The hydrolysis of lactose in to glucose and galactose by cold active -galactosidase offers a new promising approach in removal of lactose from milk to overcome the problem of lactose intolerance. Herein we propose, a 3D structure of cold active -galactosidase enzyme sourced from Pseudoalteromonas haloplanktis by using Modeler 9v8 and best model was developed having 88% of favourable region in ramachandran plot. Modelling was followed by docking studies with the help of Auto dock 4.0 against the three substrates lactose, ONPG and PNPG. In addition, comparative docking studies were also performed for the 3D model of psychrophilic -galactosidase with mesophilic and thermophilic enzymes. Docking studies revealed that binding affinity of enzyme towards the three different substrates is more for psychrophilic enzyme when compared with mesophilic and thermophilic enzymes. It indicates that the enzyme has high specific activity at low temperature when compared with mesophilic and thermophilic enzymes.

 

Keywords

Psychrozymes, -galactosidase, Ramachandran plot, Homology modeling, Docking.

 

Citation

Kumar et al. Bioinformation 6(8): 311-314 (2011)
 

Edited by

P Kangueane

 

ISSN

0973-2063

 

Publisher

Biomedical Informatics

 

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.