Title

Mapping of origin of replication in Themococcales

Authors

Krishna K Ojha¹, D Swati^{1, 2 *}

Affiliation

¹Department of Bioinformatics, MMV, Banaras Hindu University, India; ²Departments of Physics and Bioinformatics, MMV, Banaras Hindu University, India
 

Email

swatid@gmail.com

Article Type

Hypothesis

Date

Received June 7, 2010; Accepted September 24, 2010; Published November 1, 2010
 

Genome replication is a crucial and essential process for the continuity of life. In all organisms it starts at a specific region of the genome known as origin of replication (Ori) site. The number of Ori sites varies in prokaryotes and eukaryotes. Replication starts at a single Ori site in bacteria, but in eukaryotes multiple Ori sites are used for fast copying across all chromosomes. The situation becomes complex in archaea, where some groups have single and others have multiple origins of replication. Themococcales, are a hyperthermophilic order of archaea. They are anaerobes and heterotrophs-peptide fermenters, sulphate reducers, methanogens being some of the examples of metabolic types. In this paper we have applied a combination of multiple in silico approaches - Z curve, the cell division cycle (cdc6) gene location and location of consensus origin recognition box (ORB) sequences for location of origin of replication in Thermococcus onnurineus, Thermococcus gammatolerans and other Themococcales and compared the results to that of the well-documented case of Pyrococcus abyssi. The motivation behind this study is to find the number of Ori sites based on the data available for members of this order. Results from this in silico analysis show that the Themococcales have a single origin of replication.
 

Keywords

Ori sites, Disparity plot, Z curve, cdc6 gene, Intergenic repeats, ORB.

Citation

Ojha K. K. & Swati D. Bioinformation, 5 (5) 213- 218, 2010

Edited by

P. Kangueane

ISSN

0973-2063

Publisher

Biomedical Informatics

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.